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Literature summary extracted from

  • Tan, C.A.; Roberts, M.F.
    Engineering of the nonspecific phospholipase C from Bacillus cereus: Replacement of glutamic acid-4 by alanine results in loss of interfacial catalysis and enhanced phosphomonoesterase activity (1998), Biochemistry, 37, 4275-4279.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.3 transformed into Escherichia coli Bacillus cereus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.3 E4A Kunkel method Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.3 phosphatidic acid monomeric and micellar, poor substrate, inhibitor Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.3 0.03
-
 diheptanoylphosphatidylcholine micellar substrate, wild-type PLC Bacillus cereus
3.1.4.3 0.19
-
 dihexanoylphosphatidylcholine monomeric substrate, wild-type PLC Bacillus cereus
3.1.4.3 1.09
-
 diheptanoylphosphatidylcholine micellar substrate, recombinant PLC Bacillus cereus
3.1.4.3 1.12
-
 dihexanoylphosphatidylcholine monomeric substrate, recombinant PLC Bacillus cereus
3.1.4.3 4.2
-
 dihexanoylphosphatidic acid monomeric substrate, recombinant PLC Bacillus cereus
3.1.4.3 9
-
 dihexanoylphosphatidic acid monomeric substrate, wild-type PLC Bacillus cereus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.3 extracellular
-
 Bacillus cereus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.3 Zn2+ metalloenzyme Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.3 28500
-
-
 Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Bacillus cereus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3
-
 Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 diheptanoylphosphatidylcholine + H2O
-
 Bacillus cereus diheptanoylglycerol + phosphorylcholine
-
 ?
3.1.4.3 dihexanoylphosphatidic acid + H2O
-
 Bacillus cereus ?
-
 ?
3.1.4.3 dihexanoylphosphatidylcholine + H2O
-
 Bacillus cereus dihexanoylglycerol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidic acid + H2O
-
 Bacillus cereus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.3 50
-
 stable Bacillus cereus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.4.3 0.009
-
 dihexanoylphosphatidic acid monomeric substrate, wild-type PLC Bacillus cereus
3.1.4.3 0.0198
-
 dihexanoylphosphatidic acid monomeric substrate, recombinant PLC Bacillus cereus
3.1.4.3 4.93
-
 dihexanoylphosphatidylcholine monomeric substrate, recombinant PLC Bacillus cereus
3.1.4.3 6.78
-
 dihexanoylphosphatidylcholine monomeric substrate, wild-type PLC Bacillus cereus
3.1.4.3 6.85
-
 diheptanoylphosphatidylcholine micellar substrate, recombinant PLC Bacillus cereus
3.1.4.3 13.8
-
 diheptanoylphosphatidylcholine micellar substrate, wild-type PLC Bacillus cereus