Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zou, L.; Kojima, N.; Kito, M.; Yagi, K.
    Purification to homogeneity of human placental acid sphingomyelinase (1989), Biotechnol. Appl. Biochem., 11, 217-225.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.4.12 stable with n-octyl-beta-D-glucopyranoside Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.12 3-[(3-cholamidopropyl)dimethyl-ammonio]-1-propanesulfonate
-
 Homo sapiens
3.1.4.12 Brij 56
-
 Homo sapiens
3.1.4.12 cholate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.12 0.055
-
 sphingomyelin
-
 Homo sapiens
3.1.4.12 0.34
-
 2-n-hexadecanoylamino-4-nitrophenylphosphorylcholine
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.12 62000
-
 2 * 62000 SDS-PAGE Homo sapiens
3.1.4.12 123000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 sphingomyelin + H2O Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 21fold Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.12 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.12 2.8
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.1.4.12 -80°C, 50 mM n-octyl-beta-D-glucopyranoside, more than 4 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 2-n-hexadecanoylamino-4-nitrophenylphosphorylcholine + H2O
-
 Homo sapiens 2-n-hexadecanoylamino-4-nitrophenol + choline phosphate
-
 ?
3.1.4.12 sphingomyelin + H2O
-
 Homo sapiens N-acylsphingosine + choline phosphate
-
 ?
3.1.4.12 sphingomyelin + H2O
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.12 dimer 2 * 62000 SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.12 5.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.12 5 8
-
 Homo sapiens