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Literature summary extracted from

  • Griendling, K.K.; Taubman, M.B.; Akers, M.; Mendlowitz, M.; Alexander, R.W.
    Characterization of phosphatidylinositol-specific phospholipase C from cultured vascular smooth muscle cells (1991), J. Biol. Chem., 266, 15498-15504.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.11 NaCl inhibits phosphatidylinositol hydrolysis of low molecular weight PLC. Conversely, PIP2 hydrolysis is stimulated by increasing concentrations of NaCl Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.11 Ca2+ PLC requires calcium for full activation Rattus norvegicus
3.1.4.11 NaCl inhibits phosphatidylinositol hydrolysis of low molecular weight PLC. Conversely, PIP2 hydrolysis is stimulated by increasing concentrations of NaCl Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.11 58000
-
 SDS-PAGE, low molecular weight PLC hydrolyzes both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate has a molecular mass of 58 kDa. Cloning techniques have tentatively identified the low molecular weight peak as PLC-alpha Rattus norvegicus
3.1.4.11 66000
-
 low molecular PLC by size exclusion chromatography Rattus norvegicus
3.1.4.11 145000
-
 high molecular PLC shows much greater activity against phosphatidylinositol than phosphatidylinositol 4,5-bisphosphate and is active at lower calcium concentrations Rattus norvegicus
3.1.4.11 157000
-
 high molecular PLC by size exclusion chromatography Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.11 additional information Rattus norvegicus PLC activity in vascular smooth muscle cells can be divided into two types based on their calcium and pH requirements, substrate preferences, and molecular weights ?
-
 ?
3.1.4.11 additional information Rattus norvegicus several different assay conditions for both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate are tested on samples of crude supernatant for their ability to support PLC activity ?
-
 ?
3.1.4.11 additional information Rattus norvegicus the injection fraction shows no activity against phosphatidylcholine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.11 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.11 partially purified, DEAE-cellulose and heparin-Sepharose chromatographies followed by Mono Q and size exclusion HPLC Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.11 muscle cultured vascular smooth muscle cell, isolated from rat thoracic aorta Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.11 additional information
-
 PLC activity in vascular smooth muscle cells can be divided into two type based on their calcium and pH requirements, substrate preferences, and molecular weights Rattus norvegicus
3.1.4.11 28.97
-
 phosphatidylinositol as substrate, last purification step Rattus norvegicus
3.1.4.11 204.1
-
 phosphatidylinositol 4,5-bisphosphate as substrate, last purification step Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.1.4.11 Partially purified PLC can be stored in buffer C at -80°C for several months without appreciable loss of activity. However, repeated freezing and thawing has a deleterious effect on enzyme activity. More purified PLC (Mono Q and size exclusion fractions) can be stored at 4°C for 5-7 days with a gradual loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.11 additional information PLC activity in vascular smooth muscle cells can be divided into two types based on their calcium and pH requirements, substrate preferences, and molecular weights Rattus norvegicus ?
-
 ?
3.1.4.11 additional information several different assay conditions for both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate are tested on samples of crude supernatant for their ability to support PLC activity Rattus norvegicus ?
-
 ?
3.1.4.11 additional information the injection fraction shows no activity against phosphatidylcholine Rattus norvegicus ?
-
 ?
3.1.4.11 phosphatidylinositol + H2O low molecular weight PLC hydrolyzes both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate, high molecular PLC shows much greater activity against phosphatidylinositol than phosphatidylinositol 4,5-bisphosphate Rattus norvegicus ?
-
 ?
3.1.4.11 phosphatidylinositol 4,5-bisphosphate + H2O enzyme activities for phosphatidylinositol 4,5-bisphosphate are strongly dependent on the relative levels of calcium and pH in the assay buffer Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.11 phosphatidylinositol-specific phospholipase C
-
 Rattus norvegicus
3.1.4.11 PLC
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.11 5
-
 high molecular PLC (157 kDa), phosphatidylinositol as substrate Rattus norvegicus
3.1.4.11 6
-
 at the higher calcium concentration the pH optimum for phosphatidylinositol 4,5-bisphosphate hydrolysis is 6.0 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.11 5.5 7
-
 Rattus norvegicus