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Literature summary extracted from

  • Wang, P.; Toyoshima, S.; Osawa, T.
    Partial purification and characterization of membrane-bound and cytosolic phosphatidylinositol-specific phospholipases C from murine thymocytes (1986), J. Biochem., 100, 1015-1022.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.11 arachidonic acid enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme Mus musculus
3.1.4.11 diacylglycerol enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme Mus musculus
3.1.4.11 phosphatidic acid enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme Mus musculus
3.1.4.11 phosphatidylcholine enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme Mus musculus
3.1.4.11 phosphatidylethanolamine enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme Mus musculus
3.1.4.11 phosphatidylserine enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.11 cytosol
-
 Mus musculus 5829
-
3.1.4.11 membrane bound to Mus musculus 16020
-
3.1.4.11 microsome
-
 Mus musculus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.11 68000 70000 membrane-bound enzyme, in presence of sodium cholate, gel filtration Mus musculus
3.1.4.11 71000 72000 cytosolic enzyme, in presence of sodium deoxycholate, gel filtration Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.11 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.11 partial Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.11 thymocyte
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.11 0.114
-
 membrane-bound enzyme Mus musculus
3.1.4.11 0.235
-
 cytosolic enzyme Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.11 phosphatidylinositol + H2O at neutral pH and low Ca2+ concentration the membrane bound enzyme hydrolyzes phosphatidylinositol 4,5-diphosphate in preference to phosphatidylinositol Mus musculus ?
-
 ?
3.1.4.11 phosphatidylinositol 4,5-bisphosphate + H2O membrane-bound enzyme hydrolyzes phosphatidylinositol 4,5-diphosphate in preference to phosphatidylinositol Mus musculus D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.11 6
-
 hydrolysis of phosphatidylinositol, membrane bound enzyme and cytosolic enzyme Mus musculus
3.1.4.11 7.5
-
 hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme Mus musculus