BRENDA - Enzyme Database

Partial purification and characterization of membrane-bound and cytosolic phosphatidylinositol-specific phospholipases C from murine thymocytes

Wang, P.; Toyoshima, S.; Osawa, T.; J. Biochem. 100, 1015-1022 (1986)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.11
arachidonic acid
enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme
Mus musculus
3.1.4.11
diacylglycerol
enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme
Mus musculus
3.1.4.11
phosphatidic acid
enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme
Mus musculus
3.1.4.11
phosphatidylcholine
enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme
Mus musculus
3.1.4.11
phosphatidylethanolamine
enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme
Mus musculus
3.1.4.11
phosphatidylserine
enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.11
cytosol
-
Mus musculus
5829
-
3.1.4.11
membrane
bound to
Mus musculus
16020
-
3.1.4.11
microsome
-
Mus musculus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.11
68000
70000
membrane-bound enzyme, in presence of sodium cholate, gel filtration
Mus musculus
3.1.4.11
71000
72000
cytosolic enzyme, in presence of sodium deoxycholate, gel filtration
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.11
Mus musculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.11
partial
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.11
thymocyte
-
Mus musculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.11
0.114
-
membrane-bound enzyme
Mus musculus
3.1.4.11
0.235
-
cytosolic enzyme
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.11
phosphatidylinositol + H2O
at neutral pH and low Ca2+ concentration the membrane bound enzyme hydrolyzes phosphatidylinositol 4,5-diphosphate in preference to phosphatidylinositol
135123
Mus musculus
?
-
-
-
?
3.1.4.11
phosphatidylinositol 4,5-bisphosphate + H2O
membrane-bound enzyme hydrolyzes phosphatidylinositol 4,5-diphosphate in preference to phosphatidylinositol
135123
Mus musculus
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
135123
Mus musculus
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.11
6
-
hydrolysis of phosphatidylinositol, membrane bound enzyme and cytosolic enzyme
Mus musculus
3.1.4.11
7.5
-
hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.11
arachidonic acid
enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme
Mus musculus
3.1.4.11
diacylglycerol
enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme
Mus musculus
3.1.4.11
phosphatidic acid
enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme
Mus musculus
3.1.4.11
phosphatidylcholine
enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme
Mus musculus
3.1.4.11
phosphatidylethanolamine
enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme
Mus musculus
3.1.4.11
phosphatidylserine
enhances hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.11
cytosol
-
Mus musculus
5829
-
3.1.4.11
membrane
bound to
Mus musculus
16020
-
3.1.4.11
microsome
-
Mus musculus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.11
68000
70000
membrane-bound enzyme, in presence of sodium cholate, gel filtration
Mus musculus
3.1.4.11
71000
72000
cytosolic enzyme, in presence of sodium deoxycholate, gel filtration
Mus musculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.11
partial
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.11
thymocyte
-
Mus musculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.11
0.114
-
membrane-bound enzyme
Mus musculus
3.1.4.11
0.235
-
cytosolic enzyme
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.11
phosphatidylinositol + H2O
at neutral pH and low Ca2+ concentration the membrane bound enzyme hydrolyzes phosphatidylinositol 4,5-diphosphate in preference to phosphatidylinositol
135123
Mus musculus
?
-
-
-
?
3.1.4.11
phosphatidylinositol 4,5-bisphosphate + H2O
membrane-bound enzyme hydrolyzes phosphatidylinositol 4,5-diphosphate in preference to phosphatidylinositol
135123
Mus musculus
D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
135123
Mus musculus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.11
6
-
hydrolysis of phosphatidylinositol, membrane bound enzyme and cytosolic enzyme
Mus musculus
3.1.4.11
7.5
-
hyrolysis of phosphatidylinositol 4,5-diphosphate, membrane bound enzyme and cytosolic enzyme
Mus musculus