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Literature summary extracted from

  • Kobayashi, H.; Inokuchi, N.; Koyama, T.; Tomita, M.; Irie, M.
    Purification and characterization of the 2nd 5'-nucleotide-forming nuclease from Lentinus edodes (1995), Biosci. Biotechnol. Biochem., 59, 1169-1171.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.30.2 Cu2+
-
 Lentinula edodes
3.1.30.2 EDTA
-
 Lentinula edodes
3.1.30.2 Hg2+
-
 Lentinula edodes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.30.2 Zn2+ reactivates after EDTA treatment Lentinula edodes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.30.2 31000
-
 gel filtration Lentinula edodes

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.2 Lentinula edodes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.30.2 nuclease Le3 Lentinula edodes

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.30.2 fruit body
-
 Lentinula edodes
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.2 3'-nucleotides + H2O e.g. 3'-AMP Lentinula edodes ?
-
 ?
3.1.30.2 DNA + H2O heat denatured Lentinula edodes 5'-phosphonucleotides + 5'-phosphomononucleotides
-
 ?
3.1.30.2 RNA + H2O
-
 Lentinula edodes 5'-phosphooligonucleotides + 5'-phosphomononucleotides
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.30.2 5.5
-
-
 Lentinula edodes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.30.2 6 8
-
 Lentinula edodes