BRENDA - Enzyme Database

Enzymatic activities in cultured human lymphocytes that dephosphorylate dolichyl pyrophosphate and dolichyl phosphate

Wedgwood, J.F.; Strominger, J.L.; J. Biol. Chem. 255, 1120-1123 (1980)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.6.1.43
EDTA
-
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.51
phosphate
-
Homo sapiens
3.6.1.43
bacitracin
no inhibition when preincubating with EDTA
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.51
0.2
-
dolichyl phosphate
in presence of Triton X-100
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.51
plasma membrane
-
Homo sapiens
5886
-
3.6.1.43
membrane
-
Homo sapiens
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.43
additional information
no metal ions required
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.43
dolichyl diphosphate + H2O
Homo sapiens
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
dolichyl phosphate + phosphate
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.51
Homo sapiens
-
-
-
3.6.1.43
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.51
lymphocyte
-
Homo sapiens
-
3.6.1.43
lymphocyte
RPMI 4265
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.51
additional information
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.51
dolichyl phosphate + H2O
-
134908
Homo sapiens
dolichol + phosphate
-
-
-
?
3.6.1.43
dolichyl diphosphate + H2O
-
134908
Homo sapiens
dolichyl phosphate + phosphate
-
134908
Homo sapiens
?
3.6.1.43
dolichyl diphosphate + H2O
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
134908
Homo sapiens
dolichyl phosphate + phosphate
-
134908
Homo sapiens
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.51
6.5
-
-
Homo sapiens
3.6.1.43
8
-
-
Homo sapiens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.51
5
8
50% of maximal activity at pH 5 and at pH 8
Homo sapiens
3.6.1.43
7
9
95% and 90% of activity, broad pH-range
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.6.1.43
EDTA
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.51
phosphate
-
Homo sapiens
3.6.1.43
bacitracin
no inhibition when preincubating with EDTA
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.51
0.2
-
dolichyl phosphate
in presence of Triton X-100
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.51
plasma membrane
-
Homo sapiens
5886
-
3.6.1.43
membrane
-
Homo sapiens
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.43
additional information
no metal ions required
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.43
dolichyl diphosphate + H2O
Homo sapiens
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
dolichyl phosphate + phosphate
-
Homo sapiens
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.51
lymphocyte
-
Homo sapiens
-
3.6.1.43
lymphocyte
RPMI 4265
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.51
additional information
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.51
dolichyl phosphate + H2O
-
134908
Homo sapiens
dolichol + phosphate
-
-
-
?
3.6.1.43
dolichyl diphosphate + H2O
-
134908
Homo sapiens
dolichyl phosphate + phosphate
-
134908
Homo sapiens
?
3.6.1.43
dolichyl diphosphate + H2O
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
134908
Homo sapiens
dolichyl phosphate + phosphate
-
134908
Homo sapiens
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.51
6.5
-
-
Homo sapiens
3.6.1.43
8
-
-
Homo sapiens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.51
5
8
50% of maximal activity at pH 5 and at pH 8
Homo sapiens
3.6.1.43
7
9
95% and 90% of activity, broad pH-range
Homo sapiens