BRENDA - Enzyme Database

Characterization of phosphatidic acid phosphohydrolase in neutrophil subcellular fractions

Taylor, G.S.; Ladd, A.; James, J.; Greene, B.; English, D.; Biochim. Biophys. Acta 1175, 219-224 (1993)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
Triton X-100
plasma membrane-associated activity is absolutely dependent on detergent
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
F-
-
Homo sapiens
3.1.3.4
NEM
inhibition of cytosolic and light membrane activity
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.062
-
phosphatidic acid
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
cytosol
20% of the activity
Homo sapiens
5829
-
3.1.3.4
additional information
the majority of the activity is localized in particulate fraction
Homo sapiens
-
-
3.1.3.4
plasma membrane
-
Homo sapiens
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Ca2+
moderate inhibition of cytosolic and particulate activity
Homo sapiens
3.1.3.4
Mg2+
markedly potentiates the cytosolic but not the particulate activity
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
neutrophil
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
-
134887
Homo sapiens
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
Triton X-100
plasma membrane-associated activity is absolutely dependent on detergent
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
F-
-
Homo sapiens
3.1.3.4
NEM
inhibition of cytosolic and light membrane activity
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.062
-
phosphatidic acid
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
cytosol
20% of the activity
Homo sapiens
5829
-
3.1.3.4
additional information
the majority of the activity is localized in particulate fraction
Homo sapiens
-
-
3.1.3.4
plasma membrane
-
Homo sapiens
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Ca2+
moderate inhibition of cytosolic and particulate activity
Homo sapiens
3.1.3.4
Mg2+
markedly potentiates the cytosolic but not the particulate activity
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
neutrophil
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
-
134887
Homo sapiens
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?