BRENDA - Enzyme Database

Cloning and characterization of two human isozymes of Mg2+-independent phosphatidic acid phosphatase

Kai, M.; Wada, I.; Imai, S.; Sakane, F.; Kanoh, H.; J. Biol. Chem. 272, 24572-24578 (1997)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.4
cloning of two Mg2+-independent isoenzymes, PAP-2a and PAP-2b
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
additional information
post-Golgi localization
Homo sapiens
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
two Mg2+-independent isoenzymes: phosphatidic acid phosphatase PAP-1a and PAP-2b
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
32158
-
x * 32158, isoenzyme PAP-2a, calculation from nucleotide sequence
Homo sapiens
3.1.3.4
35119
-
x * 35119, isoenzyme PAP-2b, calculation from nucleotide sequence
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
additional information
Homo sapiens
critical roles of the enzyme in cell growth and differentiation
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Homo sapiens
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.1.3.4
glycoprotein
the two Mg2+-independent isoenzymes, PAP-2a and PAP-2b are glycoproteins
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
brain
-
Homo sapiens
-
3.1.3.4
colon
-
Homo sapiens
-
3.1.3.4
heart
-
Homo sapiens
-
3.1.3.4
kidney
-
Homo sapiens
-
3.1.3.4
leukocyte
peripheral blood
Homo sapiens
-
3.1.3.4
additional information
isoenzyme PAP-2b is almost ubiquitously in all human tissues, isoenzyme PAP-2a is undetectable in thymus, placenta and leukocytes
Homo sapiens
-
3.1.3.4
ovary
-
Homo sapiens
-
3.1.3.4
pancreas
-
Homo sapiens
-
3.1.3.4
placenta
-
Homo sapiens
-
3.1.3.4
prostate gland
isoenzyme PAP2a
Homo sapiens
-
3.1.3.4
skeletal muscle
-
Homo sapiens
-
3.1.3.4
small intestine
-
Homo sapiens
-
3.1.3.4
spleen
-
Homo sapiens
-
3.1.3.4
testis
-
Homo sapiens
-
3.1.3.4
thymus
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
ceramide-1-phosphate + H2O
isoenzyme PAP-2a and PAP-2b
134884
Homo sapiens
?
-
-
-
?
3.1.3.4
lysophosphatidate + H2O
isoenzyme PAP-2a and PAP-2b
134884
Homo sapiens
?
-
-
-
?
3.1.3.4
additional information
critical roles of the enzyme in cell growth and differentiation
134884
Homo sapiens
?
-
-
-
-
3.1.3.4
phosphatidic acid + H2O
-
134884
Homo sapiens
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
sphingosine 1-phosphate + H2O
hydrolysis by isoenzyme PAP-2b, no hydrolysis by isoenzyme PAP-2a
134884
Homo sapiens
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.4
?
x * 32158, isoenzyme PAP-2a, calculation from nucleotide sequence; x * 35119, isoenzyme PAP-2b, calculation from nucleotide sequence
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
cloning of two Mg2+-independent isoenzymes, PAP-2a and PAP-2b
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
additional information
post-Golgi localization
Homo sapiens
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
two Mg2+-independent isoenzymes: phosphatidic acid phosphatase PAP-1a and PAP-2b
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
32158
-
x * 32158, isoenzyme PAP-2a, calculation from nucleotide sequence
Homo sapiens
3.1.3.4
35119
-
x * 35119, isoenzyme PAP-2b, calculation from nucleotide sequence
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
additional information
Homo sapiens
critical roles of the enzyme in cell growth and differentiation
?
-
-
-
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.1.3.4
glycoprotein
the two Mg2+-independent isoenzymes, PAP-2a and PAP-2b are glycoproteins
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
brain
-
Homo sapiens
-
3.1.3.4
colon
-
Homo sapiens
-
3.1.3.4
heart
-
Homo sapiens
-
3.1.3.4
kidney
-
Homo sapiens
-
3.1.3.4
leukocyte
peripheral blood
Homo sapiens
-
3.1.3.4
additional information
isoenzyme PAP-2b is almost ubiquitously in all human tissues, isoenzyme PAP-2a is undetectable in thymus, placenta and leukocytes
Homo sapiens
-
3.1.3.4
ovary
-
Homo sapiens
-
3.1.3.4
pancreas
-
Homo sapiens
-
3.1.3.4
placenta
-
Homo sapiens
-
3.1.3.4
prostate gland
isoenzyme PAP2a
Homo sapiens
-
3.1.3.4
skeletal muscle
-
Homo sapiens
-
3.1.3.4
small intestine
-
Homo sapiens
-
3.1.3.4
spleen
-
Homo sapiens
-
3.1.3.4
testis
-
Homo sapiens
-
3.1.3.4
thymus
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
ceramide-1-phosphate + H2O
isoenzyme PAP-2a and PAP-2b
134884
Homo sapiens
?
-
-
-
?
3.1.3.4
lysophosphatidate + H2O
isoenzyme PAP-2a and PAP-2b
134884
Homo sapiens
?
-
-
-
?
3.1.3.4
additional information
critical roles of the enzyme in cell growth and differentiation
134884
Homo sapiens
?
-
-
-
-
3.1.3.4
phosphatidic acid + H2O
-
134884
Homo sapiens
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
sphingosine 1-phosphate + H2O
hydrolysis by isoenzyme PAP-2b, no hydrolysis by isoenzyme PAP-2a
134884
Homo sapiens
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.4
?
x * 32158, isoenzyme PAP-2a, calculation from nucleotide sequence; x * 35119, isoenzyme PAP-2b, calculation from nucleotide sequence
Homo sapiens