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Literature summary extracted from

  • Ide, H.; Nakazawa, Y.
    Phosphatidate phosphatase in rat liver: the relationship between the activities with membrane-bound phosphatidate and aqueous dispersion of phosphatidate (1985), J. Biochem., 97, 45-54.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.4 Tween 20 increases hydrolysis of phosphatidate bound to microsomal membrane or phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes, little effect on hydrolysis of phosphatidate dispersed in sonicated microsomal lipid Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.4 EDTA inhibition of hydrolysis of phosphatidate bound to microsomal membrane or phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes, no effect on hydrolysis of phosphatidate dispersed in sonicated microsomal lipid Rattus norvegicus
3.1.3.4 Tween 20 hydrolysis of phosphatidate bound to microsomal membrane in presence of optimal concentration of 8 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.4 0.1
-
 phosphatidic acid phosphatidate dispersed in sonicated microsomal lipid Rattus norvegicus
3.1.3.4 0.16
-
 phosphatidic acid phosphatidate bound to microsomal membrane Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.4 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.4 Mg2+ stimulates hydrolysis of phosphatidate bound to microsomal membrane or phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes, optimal concentration: 8 mM, no effect on hydrolysis of phosphatidate dispersed in sonicated microsomal lipid Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.4 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.4 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.4 phosphatidic acid + H2O phosphatidate bound to microsomal membrane, phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes or phosphatidate dispersed in sonicated microsomal lipid Rattus norvegicus 1,2-diacyl-sn-glycerol + phosphate
-
 ?