BRENDA - Enzyme Database

Phosphatidate phosphatase in rat liver: the relationship between the activities with membrane-bound phosphatidate and aqueous dispersion of phosphatidate

Ide, H.; Nakazawa, Y.; J. Biochem. 97, 45-54 (1985)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
Tween 20
increases hydrolysis of phosphatidate bound to microsomal membrane or phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes, little effect on hydrolysis of phosphatidate dispersed in sonicated microsomal lipid
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
EDTA
inhibition of hydrolysis of phosphatidate bound to microsomal membrane or phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes, no effect on hydrolysis of phosphatidate dispersed in sonicated microsomal lipid
Rattus norvegicus
3.1.3.4
Tween 20
hydrolysis of phosphatidate bound to microsomal membrane in presence of optimal concentration of 8 mM
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.1
-
phosphatidic acid
phosphatidate dispersed in sonicated microsomal lipid
Rattus norvegicus
3.1.3.4
0.16
-
phosphatidic acid
phosphatidate bound to microsomal membrane
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
cytosol
-
Rattus norvegicus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
stimulates hydrolysis of phosphatidate bound to microsomal membrane or phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes, optimal concentration: 8 mM, no effect on hydrolysis of phosphatidate dispersed in sonicated microsomal lipid
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
phosphatidate bound to microsomal membrane, phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes or phosphatidate dispersed in sonicated microsomal lipid
134864
Rattus norvegicus
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
Tween 20
increases hydrolysis of phosphatidate bound to microsomal membrane or phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes, little effect on hydrolysis of phosphatidate dispersed in sonicated microsomal lipid
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
EDTA
inhibition of hydrolysis of phosphatidate bound to microsomal membrane or phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes, no effect on hydrolysis of phosphatidate dispersed in sonicated microsomal lipid
Rattus norvegicus
3.1.3.4
Tween 20
hydrolysis of phosphatidate bound to microsomal membrane in presence of optimal concentration of 8 mM
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.1
-
phosphatidic acid
phosphatidate dispersed in sonicated microsomal lipid
Rattus norvegicus
3.1.3.4
0.16
-
phosphatidic acid
phosphatidate bound to microsomal membrane
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
cytosol
-
Rattus norvegicus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
stimulates hydrolysis of phosphatidate bound to microsomal membrane or phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes, optimal concentration: 8 mM, no effect on hydrolysis of phosphatidate dispersed in sonicated microsomal lipid
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
phosphatidate bound to microsomal membrane, phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes or phosphatidate dispersed in sonicated microsomal lipid
134864
Rattus norvegicus
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?