BRENDA - Enzyme Database

Phosphatidate phosphatase activity in isolated rod outer segment from bovine retina

Pasquare de Garcia, S.J.; Giusto, N.M.; Biochim. Biophys. Acta 875, 195-202 (1986)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
taurocholate
stimulates
Bos taurus
3.1.3.4
Triton X-100
stimulates
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
Ca2+
1 mM, 10% inhibition
Bos taurus
3.1.3.4
EDTA
50 mM, 70% inhibition
Bos taurus
3.1.3.4
F-
50 mM, 80% inhibition
Bos taurus
3.1.3.4
Mg2+
2.5 mM, slight inhibition
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.67
-
phosphatidic acid
-
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
microsome
-
Bos taurus
-
-
3.1.3.4
mitochondrion
-
Bos taurus
5739
-
3.1.3.4
plasma membrane
associated with disk membrane, photoreceptor membrane of bovine retina
Bos taurus
5886
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Bos taurus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
retina
rod outer segment
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
-
134862
Bos taurus
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
7.5
-
-
Bos taurus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.4
6
8
pH 6.0: about 40% of maximal activity, pH 8.0: about 25% of maximal activity
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
taurocholate
stimulates
Bos taurus
3.1.3.4
Triton X-100
stimulates
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
Ca2+
1 mM, 10% inhibition
Bos taurus
3.1.3.4
EDTA
50 mM, 70% inhibition
Bos taurus
3.1.3.4
F-
50 mM, 80% inhibition
Bos taurus
3.1.3.4
Mg2+
2.5 mM, slight inhibition
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.67
-
phosphatidic acid
-
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
microsome
-
Bos taurus
-
-
3.1.3.4
mitochondrion
-
Bos taurus
5739
-
3.1.3.4
plasma membrane
associated with disk membrane, photoreceptor membrane of bovine retina
Bos taurus
5886
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
retina
rod outer segment
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
-
134862
Bos taurus
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
7.5
-
-
Bos taurus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.4
6
8
pH 6.0: about 40% of maximal activity, pH 8.0: about 25% of maximal activity
Bos taurus