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Literature summary extracted from

  • Pontremoli, S.; Traniello, S.
    Fructose-1,6-diphosphatase and sedoheptulose-1,7-diphosphatase from Candida utilis (1975), Methods Enzymol., 42C, 347-353.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.11 CoA activates Cyberlindnera jadinii
3.1.3.11 cystamine activates Cyberlindnera jadinii
3.1.3.37 EDTA increases activity Cyberlindnera jadinii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.11 AMP
-
 Cyberlindnera jadinii
3.1.3.11 fructose 1,6-diphosphate above 0.1 mM Cyberlindnera jadinii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 0.008
-
 fructose 1,6-diphosphate
-
 Cyberlindnera jadinii
3.1.3.37 1
-
 sedoheptulose 1,7-bisphosphate at pH 6.0 Cyberlindnera jadinii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.11 Mg2+ divalent cation required. Mn2+ or Mg2+ Cyberlindnera jadinii
3.1.3.11 Mn2+ divalent cation required. Mn2+ or Mg2+ Cyberlindnera jadinii
3.1.3.11 Mn2+ less active in activation than Mg2+ Cyberlindnera jadinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.11 35000
-
 4 * 35000, SDS-PAGE Cyberlindnera jadinii
3.1.3.11 130000
-
 sucrose density gradient centrifugation Cyberlindnera jadinii
3.1.3.37 35000
-
 2 * 35000, SDS-PAGE Cyberlindnera jadinii
3.1.3.37 75000
-
 sucrose density gradient centrifugation Cyberlindnera jadinii

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Cyberlindnera jadinii
-
-
-
3.1.3.37 Cyberlindnera jadinii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11
-
 Cyberlindnera jadinii
3.1.3.37
-
 Cyberlindnera jadinii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.11 73
-
-
 Cyberlindnera jadinii
3.1.3.37 11.5
-
-
 Cyberlindnera jadinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-diphosphate + H2O
-
 Cyberlindnera jadinii D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 sedoheptulose 1,7-bisphosphate + H2O no activity Cyberlindnera jadinii sedoheptulose 7-phosphate + phosphate
-
 ?
3.1.3.37 sedoheptulose 1,7-bisphosphate + H2O
-
 Cyberlindnera jadinii sedoheptulose 7-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.11 tetramer 4 * 35000, SDS-PAGE Cyberlindnera jadinii
3.1.3.37 dimer 2 * 35000, SDS-PAGE Cyberlindnera jadinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.11 8.6 8.8
-
 Cyberlindnera jadinii
3.1.3.37 6
-
-
 Cyberlindnera jadinii