Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wojcieszyn, J.W.; Wang, M.C.; Lee, C.L.; Murphy, G.P.; Chu, T.M.
    Purification and characterization of a human urinary acid phosphatase (1979), J. Appl. Biochem., 1, 223-234.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 F-
-
 Homo sapiens
3.1.3.2 Fe2+
-
 Homo sapiens
3.1.3.2 Mg2+
-
 Homo sapiens
3.1.3.2 phosphate
-
 Homo sapiens
3.1.3.2 Tartrate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.1
-
 Alpha-naphthyl phosphate urinary enzyme and prostatic enzyme Homo sapiens
3.1.3.2 0.36
-
 p-nitrophenyl phosphate prostatic enzyme Homo sapiens
3.1.3.2 0.52
-
 p-nitrophenyl phosphate urinary enzyme Homo sapiens
3.1.3.2 1
-
 AMP urinary enzyme Homo sapiens
3.1.3.2 2
-
 AMP prostatic enzyme Homo sapiens
3.1.3.2 3.3
-
 phosphorylcholine prostatic enzyme Homo sapiens
3.1.3.2 3.3
-
 thymolphthalein monophosphate urinary enzyme Homo sapiens
3.1.3.2 4.6
-
 phosphoryl choline urinary enzyme Homo sapiens
3.1.3.2 5
-
 thymolphthalein monophosphate prostatic enzyme Homo sapiens
3.1.3.2 45.3
-
 2-glycerophosphate prostatic enzyme Homo sapiens
3.1.3.2 66.7
-
 2-glycerophosphate urinary enzyme Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 48000
-
 2 * 48000, urinary enzyme, SDS-PAGE Homo sapiens
3.1.3.2 97000
-
 urinary enzyme, sucrose density gradient ultracentrifugation Homo sapiens
3.1.3.2 100000
-
 urinary enzyme, gel filtration, native gradient PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 isoenzyme A, B and C 103 Homo sapiens
3.1.3.2 urine Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 prostate gland
-
 Homo sapiens
-
3.1.3.2 urine female Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 333
-
 urinary enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 1-naphthyl phosphate + H2O
-
 Homo sapiens 1-naphthol + phosphate
-
 ?
3.1.3.2 2-glycerophosphate + H2O
-
 Homo sapiens glycerol + phosphate
-
 ?
3.1.3.2 AMP + H2O
-
 Homo sapiens adenosine + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Homo sapiens p-nitrophenol + phosphate
-
 ?
3.1.3.2 phosphocholine + H2O
-
 Homo sapiens choline + phosphate
-
 ?
3.1.3.2 thymolphthalein monophosphate + H2O
-
 Homo sapiens thymolphthalein + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 dimer 2 * 48000, urinary enzyme, SDS-PAGE Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 37
-
 2 h, 39% loss of activity of urinary enzyme, 88% loss of activity of prostatic enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5.8
-
 urinary enzyme Homo sapiens