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Literature summary extracted from

  • Ching, T.M.; Lin, T.P.; Metzger, R.J.
    Purification and properties of acid phosphatase from plump and shriveled seeds of triticale (1987), Plant Physiol., 84, 789-795.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.2 ascorbate 10 mM, 1.8-2fold activation of ATP hydrolysis Secale cereale x Triticum aestivum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 ascorbate 10 mM, hydrolysis of p-nitrophenyl phosphate Secale cereale x Triticum aestivum
3.1.3.2 citrate 40 mM, 30% inhibition of ATP hydrolysis of enzyme from plump seed Secale cereale x Triticum aestivum
3.1.3.2 Cu2+ 1 mM, hydrolysis of p-nitrophenyl phosphate Secale cereale x Triticum aestivum
3.1.3.2 EDTA 10 mM Secale cereale x Triticum aestivum
3.1.3.2 F- 5 mM NaF Secale cereale x Triticum aestivum
3.1.3.2 FeSO4
-
 Secale cereale x Triticum aestivum
3.1.3.2 Hg2+ 1 mM, inhibition of hydrolysis of p-nitrophenyl phosphate, slight increase of ATP hydrolysis Secale cereale x Triticum aestivum
3.1.3.2 molybdate
-
 Secale cereale x Triticum aestivum
3.1.3.2 Pb(CH3COO)2 10 mM, strong inhibition of enzyme from plump seed, weak inhibition of enzyme from shriveled seed, hydrolysis of p-nitrophenyl phosphate and ATP Secale cereale x Triticum aestivum
3.1.3.2 phosphate at 1 mM or above, enzyme from plump seed Secale cereale x Triticum aestivum
3.1.3.2 Tartrate 10 mM, slight inhibition of hydrolysis of p-nitrophenyl phosphate with enzyme from plump seed, slight activation of ATP hydrolysis, enzyme from plump and shriveled seed Secale cereale x Triticum aestivum
3.1.3.2 Zn2+ 1 mM, hydrolysis of p-nitrophenyl phosphate Secale cereale x Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.32
-
 ATP enzyme from plump seed Secale cereale x Triticum aestivum
3.1.3.2 0.35
-
 p-nitrophenyl phosphate enzyme from shriveled seed Secale cereale x Triticum aestivum
3.1.3.2 0.5
-
 ATP enzyme from shriveled seed Secale cereale x Triticum aestivum
3.1.3.2 0.57
-
 p-nitrophenyl phosphate enzyme from plump seed Secale cereale x Triticum aestivum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Ca2+ 10 mM CaCl2, slight activation of hydrolysis of ATP, 2.3fold increase of activity with p-nitrophenyl phosphate Secale cereale x Triticum aestivum
3.1.3.2 Cu2+ 1 mM CuSO4, slight activation of ATP hydrolysis Secale cereale x Triticum aestivum
3.1.3.2 Mg2+ 10 mM MgCl2, 2-3fold increase of hydrolysis of p-nitrophenyl phosphate, slight increase of hydrolysis of ATP Secale cereale x Triticum aestivum
3.1.3.2 Zn2+ 1 mM ZnSO4, 1.4-1.5fold activation of ATP hydrolysis Secale cereale x Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Secale cereale x Triticum aestivum
-
 X Triticosecale Wittmarck
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.2 glycoprotein contains 12% carbohydrate Secale cereale x Triticum aestivum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2
-
 Secale cereale x Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 seed shriveled and plump Secale cereale x Triticum aestivum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 ADP + H2O 19% of the activity with p-nitrophenyl phosphate, enzyme from plump seed. 33% of the activity with p-nitrophenyl phosphate, enzyme from shriveled seed Secale cereale x Triticum aestivum AMP + phosphate
-
 ?
3.1.3.2 ATP + H2O 62% of the activity with p-nitrophenyl phosphate, enzyme from plump seed. 64% of the activity with p-nitrophenyl phosphate, enzyme from shriveled seed Secale cereale x Triticum aestivum ADP + phosphate
-
 ?
3.1.3.2 diphosphate + H2O 86% of the activity with p-nitrophenyl phosphate, enzyme from plump seed. 84% of the activity with p-nitrophenyl phosphate, enzyme from shriveled seed Secale cereale x Triticum aestivum 2 phosphate
-
 ?
3.1.3.2 GTP + H2O 74% of the activity with p-nitrophenyl phosphate, enzyme from plump seed. 69% of the activity with p-nitrophenyl phosphate, enzyme from shriveled seed Secale cereale x Triticum aestivum GDP + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Secale cereale x Triticum aestivum p-nitrophenol + phosphate
-
 ?
3.1.3.2 UTP + 2 H2O 62% of the activity with p-nitrophenyl phosphate, enzyme from plump seed. 64% of the activity with p-nitrophenyl phosphate, enzyme from shriveled seed Secale cereale x Triticum aestivum UMP + 2 phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 4.5
-
 hydrolysis of ATP, enzyme from plump seed Secale cereale x Triticum aestivum
3.1.3.2 4.8
-
 hydrolysis of p-nitrophenyl phosphate Secale cereale x Triticum aestivum
3.1.3.2 5.5
-
 hydrolysis of ATP, enzyme from shriveled seed Secale cereale x Triticum aestivum