BRENDA - Enzyme Database show

Periplasmic nonspecific acid phosphatase II from Salmonella typhimurium LT2. Crystallization, detergent reactivation, and phosphotransferase activity

Uerkvitz, W.; J. Biol. Chem. 263, 15823-15830 (1988)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
3.1.3.2
-
Salmonella enterica subsp. enterica serovar Typhimurium
General Stability
EC Number
General Stability
Organism
3.1.3.2
polyethylene glycol, non-ionic, or Zwitterionic detergents are indispensable for enzyme stability
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.2
2'-deoxyribonucleosides
-
Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2
thymidine
-
Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.2
24000
-
4 * 24000, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.2
Salmonella enterica subsp. enterica serovar Typhimurium
-
LT12
-
3.1.3.2
Salmonella enterica subsp. enterica serovar Typhimurium LT12
-
LT12
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.2
-
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.2
p-nitrophenyl phosphate + 2'-deoxyadenosine
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + 2'-deoxycytidine
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + 2'-deoxyuridine
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + 2-mercaptoethanol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + 2-mercaptoethanol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium LT12
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + adenosine
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + ethanol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
p-nitrophenyl phosphate + ethylphosphate
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + ethanol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium LT12
p-nitrophenyl phosphate + ethylphosphate
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + ethylene glycol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + ethylene glycol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium LT12
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + glycerol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + glycerol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium LT12
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + methanol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
p-nitrophenol + methylphosphate
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + methanol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium LT12
p-nitrophenol + methylphosphate
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + uridine
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.2
tetramer
4 * 24000, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.1.3.2
-
Salmonella enterica subsp. enterica serovar Typhimurium
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.3.2
polyethylene glycol, non-ionic, or Zwitterionic detergents are indispensable for enzyme stability
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.2
2'-deoxyribonucleosides
-
Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2
thymidine
-
Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.2
24000
-
4 * 24000, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.2
-
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.2
p-nitrophenyl phosphate + 2'-deoxyadenosine
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + 2'-deoxycytidine
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + 2'-deoxyuridine
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + 2-mercaptoethanol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + 2-mercaptoethanol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium LT12
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + adenosine
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + ethanol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
p-nitrophenyl phosphate + ethylphosphate
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + ethanol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium LT12
p-nitrophenyl phosphate + ethylphosphate
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + ethylene glycol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + ethylene glycol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium LT12
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + glycerol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + glycerol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium LT12
?
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + methanol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
p-nitrophenol + methylphosphate
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + methanol
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium LT12
p-nitrophenol + methylphosphate
-
-
-
?
3.1.3.2
p-nitrophenyl phosphate + uridine
phosphotransferase activity
134701
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.2
tetramer
4 * 24000, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium