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Literature summary extracted from
- Enterobacter ribonuclease. II. A reexamination of the enzymes specificity toward internucleotide linkages (1971), Biochim. Biophys. Acta, 246, 476-486.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 4.6.1.21 | analysis | - |
Enterobacter sp. |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.21 | 24300 | 25500 | gel filtration, MW depending on purification procedure | Enterobacter sp. |
| 4.6.1.21 | 28500 | 31500 | sucrose density gradient centrifugation, MW depending on purification procedure | Enterobacter sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.6.1.21 | RNA + H2O | Enterobacter sp. | preferential cleavage at CpA | nucleoside 3'-phosphates and 3'-phosphooligonucleotides and 2',3'-cyclic phosphates | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.6.1.21 | Enterobacter sp. | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.6.1.21 | - |
Enterobacter sp. |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.6.1.21 | -20°C, 0.1 mg protein per ml Tris-HCL elution buffer, stable for about 3 weeks | Enterobacter sp. |
| 4.6.1.21 | -20°C, lyophilized enzyme, stable for about 3 weeks | Enterobacter sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.6.1.21 | RNA + H2O | preferential cleavage at CpA | Enterobacter sp. | nucleoside 3'-phosphates and 3'-phosphooligonucleotides and 2',3'-cyclic phosphates | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.21 | 55 | - |
for 15 min, 100% activity | Enterobacter sp. |
| 4.6.1.21 | 65 | - |
for 15 min, 78-80% activity depending on purification procedure | Enterobacter sp. |
| 4.6.1.21 | 75 | - |
for 15 min, 53-57% activity depending on purification procedure | Enterobacter sp. |
| 4.6.1.21 | 85 | - |
for 15 min, 18-20% activity depending on purification procedure | Enterobacter sp. |
| 4.6.1.21 | 100 | - |
for 15 min, total loss of activity | Enterobacter sp. |
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Release 2023.1 (January 2023)
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