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Literature summary extracted from

  • Fujii, T.; Doi, Y.; Ueno, H.; Hayashi, R.
    Effect of mutagenic replacement of the carboxyl terminal amino acid, val124, on the properties and regeneration of bovine pancreatic ribonuclease A (2000), J. Biochem., 127, 877-881.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.18 expression of mutants in Escherichia coli Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.18 V124A C-terminus involved in the formation of disulfide bonds during refolding process Bos taurus
4.6.1.18 V124E C-terminus involved in the formation of disulfide bonds during refolding process Bos taurus
4.6.1.18 V124G C-terminus involved in the formation of disulfide bonds during refolding process Bos taurus
4.6.1.18 V124K C-terminus involved in the formation of disulfide bonds during refolding process Bos taurus
4.6.1.18 V124L C-terminus involved in the formation of disulfide bonds during refolding process Bos taurus
4.6.1.18 V124W C-terminus involved in the formation of disulfide bonds during refolding process Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.18 0.46 0.71 poly(C) wild type and mutant enzyme Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.18 RNA + H2O Bos taurus endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.18 of the mutant proteins Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.18 pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.18 cyclic 2',3'-nucleoside monophosphate + H2O second step of hydrolysis is irreversible Bos taurus 3'-phosphomononucleotides
-
 ir
4.6.1.18 poly (C) + H2O
-
 Bos taurus 3'-CMP + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 RNA + H2O endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA Bos taurus 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 RNA + H2O first step of hydrolysis is reversible Bos taurus cyclic 2',3'-nucleoside monophosphate
-
 r