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Literature summary extracted from

  • Bartholeyns, J.; Baudhuin, P.
    Purification of rat liver particulate neutral ribonuclease and comparison of properties with pancreas and serum ribonucleases (1977), Biochem. J., 163, 675-683.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
4.6.1.18 24 h, 125 mM H2SO4, no loss of activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.18 liver natural inhibitor
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.6.1.18 lysosome
-
 Rattus norvegicus 5764
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.18 RNA + H2O Rattus norvegicus endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.18
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.18 liver
-
 Rattus norvegicus
-
4.6.1.18 serum
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.18 cyclic 2',3'-nucleoside monophosphate + H2O second step of hydrolysis is irreversible Rattus norvegicus 3'-phosphomononucleotides
-
 ir
4.6.1.18 RNA + H2O endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA Rattus norvegicus 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 RNA + H2O specific for pyrimidine bases Rattus norvegicus 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 RNA + H2O first step of hydrolysis is reversible Rattus norvegicus cyclic 2',3'-nucleoside monophosphate
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.6.1.18 60
-
 no loss of activity after 50 min, pH 5 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.18 7.7
-
-
 Rattus norvegicus
4.6.1.18 8
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.6.1.18 6 10 pH 6: about 15% of activity maximum, pH 10: about 10% of activity maximum Rattus norvegicus