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Literature summary extracted from

  • Robertson, H.D.; Altman, S.; Smith, J.D.
    Purification and properties of a specific Escherichia coli ribonuclease which cleaves a tyrosine transfer ribonucleic acid precursor (1972), J. Biol. Chem., 247, 5243-5251.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.26.7 NH4+ stimulates in presence of Mg2+ Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.26.7 Na+
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.26.5 ribosome
-
 Escherichia coli 5840
-
3.1.26.7 ribosome
-
 Escherichia coli 5840
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.5 additional information requires a monovalent and a divalent cation for activity Escherichia coli
3.1.26.7 K+ stimulates in presence of Mg2+ Escherichia coli
3.1.26.7 Mg2+ requires divalent cations Escherichia coli
3.1.26.7 Mn2+ may partially replace Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.26.7 tRNA precursor + H2O Escherichia coli
-
 tRNA + 5'-terminal oligonucleotide
-
 ?
3.1.26.7 tRNA precursor + H2O Escherichia coli MRE 600
-
 tRNA + 5'-terminal oligonucleotide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.5 Escherichia coli
-
-
-
3.1.26.5 Escherichia coli MRE 600
-
-
-
3.1.26.7 Escherichia coli
-
-
-
3.1.26.7 Escherichia coli MRE 600
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.5 partial Escherichia coli
3.1.26.7 partial Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.5 phage f2RNA + H2O degradation to a more limited extent than tRNA precursor Escherichia coli ?
-
 ?
3.1.26.5 phage f2RNA + H2O degradation to a more limited extent than tRNA precursor Escherichia coli MRE 600 ?
-
 ?
3.1.26.5 phi80-induced RNA + H2O degradation to a more limited extent than tRNA precursor Escherichia coli ?
-
 ?
3.1.26.5 phi80-induced RNA + H2O degradation to a more limited extent than tRNA precursor Escherichia coli MRE 600 ?
-
 ?
3.1.26.5 pre-tRNA precursor + H2O the enzyme cleaves only a single phosphodiester bond of the 129 nucleotide tyrosine tRNA precursor molecule. This cleavage removes all extra nucleotides present at the 5'-terminus of the precursor as a 41 nucleotide fragment, exposing the 5'-end of the mature tRNA Escherichia coli tRNA + 5'-oligoribonucleotide
-
 ?
3.1.26.5 pre-tRNA precursor + H2O pre-tRNATyr Escherichia coli tRNA + 5'-oligoribonucleotide
-
 ?
3.1.26.5 pre-tRNA precursor + H2O the enzyme cleaves only a single phosphodiester bond of the 129 nucleotide tyrosine tRNA precursor molecule. This cleavage removes all extra nucleotides present at the 5'-terminus of the precursor as a 41 nucleotide fragment, exposing the 5'-end of the mature tRNA Escherichia coli MRE 600 tRNA + 5'-oligoribonucleotide
-
 ?
3.1.26.5 pre-tRNA precursor + H2O pre-tRNATyr Escherichia coli MRE 600 tRNA + 5'-oligoribonucleotide
-
 ?
3.1.26.7 tRNA precursor + H2O
-
 Escherichia coli tRNA + 5'-terminal oligonucleotide
-
 ?
3.1.26.7 tRNA precursor + H2O 5'-end sequence is pGGUGGGG Escherichia coli tRNA + 5'-terminal oligonucleotide
-
 ?
3.1.26.7 tRNA precursor + H2O
-
 Escherichia coli MRE 600 tRNA + 5'-terminal oligonucleotide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.26.7 RNase P
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.5 8
-
-
 Escherichia coli
3.1.26.7 8
-
-
 Escherichia coli