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Literature summary extracted from

  • Buesen, W.
    Purification, subunit structure, and serological analysis of calf thymus ribonuclease H I (1980), J. Biol. Chem., 255, 9434-9443.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.26.4 (NH4)2SO4 ribonuclease H I acts optimally at about 30 mM (NH4)2SO4 in the case of Mn2+ activation, in the presence of Mg2+ ions the enzyme acts best if (NH4)2SO4 is omitted Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.26.4 NEM the Mg2+-dependent activity is inhibited by 60% at 20 mM. The Mn2+-dependent activity is unaffected Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.26.4 nucleus ribonuclease H I Bos taurus 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.4 Mg2+ optimal activity at 10 mM Bos taurus
3.1.26.4 Mn2+ optimal activity at 1 mM Bos taurus
3.1.26.4 additional information the enzyme exists in two different conformations depending on the type of divalent cation activation Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.4 24300
-
 1 * 31600, A, + 2 * 26600, B, + 1 * 24800, C, + 1 * 24300, D, SDS-PAGE Bos taurus
3.1.26.4 24800
-
 1 * 31600, A, + 2 * 26600, B, + 1 * 24800, C, + 1 * 24300, D, SDS-PAGE Bos taurus
3.1.26.4 26600
-
 1 * 31600, A, + 2 * 26600, B, + 1 * 24800, C, + 1 * 24300, D, SDS-PAGE Bos taurus
3.1.26.4 31600
-
 1 * 31600, A, + 2 * 26600, B, + 1 * 24800, C, + 1 * 24300, D, SDS-PAGE Bos taurus
3.1.26.4 80000
-
 calculation from sedimentation behavior Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.4 Bos taurus
-
 calf
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.4 large scale purification Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.26.4 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.26.4 additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.4 RNA-DNA hybrid + H2O
-
 Bos taurus ribonucleotide 5'-phosphomonoester
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.26.4 tetramer 1 * 31600, A, + 2 * 26600, B, + 1 * 24800, C, + 1 * 24300, D, SDS-PAGE Bos taurus