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Literature summary extracted from
- Ribonuclease III (1982), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 15, 485-499.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.3 | NH4+ | - |
Mus musculus |  |
| 3.1.26.3 | NH4+ | maximum activity at 0.15-0.3 M | Escherichia coli | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.3 | Co2+ | - |
Escherichia coli | |
| 3.1.26.3 | Cu2+ | - |
Escherichia coli | |
| 3.1.26.3 | Ethidium bromide | at 1.5 M | Escherichia coli | |
| 3.1.26.3 | Ethidium bromide | - |
Homo sapiens | |
| 3.1.26.3 | Ethidium bromide | - |
Mus musculus | |
| 3.1.26.3 | Fe3+ | - |
Escherichia coli | |
| 3.1.26.3 | Hg2+ | - |
Escherichia coli | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.26.3 | Co2+ | inhibits | Escherichia coli | |
| 3.1.26.3 | K+ | - |
Mus musculus | |
| 3.1.26.3 | K+ | stimulates, maximum activity at 0.15-0.3 M | Escherichia coli | |
| 3.1.26.3 | Mg2+ | required | Mus musculus | |
| 3.1.26.3 | Mg2+ | required | Escherichia coli | |
| 3.1.26.3 | Mg2+ | required | Homo sapiens | |
| 3.1.26.3 | Mn2+ | required | Escherichia coli | |
| 3.1.26.3 | Na+ | stimulates, maximum activity at 0.15-0.3 M | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.3 | 40000 | 55000 | sucrose density gradient centrifugation, gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.3 | double-stranded RNA + H2O | Mus musculus | cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA | 5'-phosphooligonucleotides | - |
? | |
| 3.1.26.3 | double-stranded RNA + H2O | Escherichia coli | cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA | 5'-phosphooligonucleotides | - |
? | |
| 3.1.26.3 | double-stranded RNA + H2O | Homo sapiens | cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA | 5'-phosphooligonucleotides | - |
? | |
| 3.1.26.3 | double-stranded RNA + H2O | Bos taurus | cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA | 5'-phosphooligonucleotides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.26.3 | Bos taurus | - |
- |
- |
| 3.1.26.3 | Escherichia coli | - |
- |
- |
| 3.1.26.3 | Homo sapiens | - |
- |
- |
| 3.1.26.3 | Mus musculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.26.3 | - |
Escherichia coli |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.26.3 | HeLa cell | - |
Homo sapiens | - |
| 3.1.26.3 | Krebs II ascites cell | - |
Mus musculus | - |
| 3.1.26.3 | thymus | - |
Bos taurus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.3 | double-stranded RNA + H2O | - |
Mus musculus | 5'-phosphooligonucleotides | - |
? | |
| 3.1.26.3 | double-stranded RNA + H2O | - |
Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
| 3.1.26.3 | double-stranded RNA + H2O | - |
Homo sapiens | 5'-phosphooligonucleotides | - |
? | |
| 3.1.26.3 | double-stranded RNA + H2O | - |
Bos taurus | 5'-phosphooligonucleotides | - |
? | |
| 3.1.26.3 | double-stranded RNA + H2O | cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA | Mus musculus | 5'-phosphooligonucleotides | - |
? | |
| 3.1.26.3 | double-stranded RNA + H2O | cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA | Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
| 3.1.26.3 | double-stranded RNA + H2O | cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA | Homo sapiens | 5'-phosphooligonucleotides | - |
? | |
| 3.1.26.3 | double-stranded RNA + H2O | cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA | Bos taurus | 5'-phosphooligonucleotides | - |
? | |
| 3.1.26.3 | single-stranded RNA + H2O | - |
Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
| 3.1.26.3 | single-stranded RNA + H2O | - |
Homo sapiens | 5'-phosphooligonucleotides | - |
? | |
| 3.1.26.3 | single-stranded RNA + H2O | - |
Bos taurus | 5'-phosphooligonucleotides | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.26.3 | dimer | - |
Mus musculus |
| 3.1.26.3 | dimer | - |
Escherichia coli |
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