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Literature summary extracted from

  • Waterborg, J.H.; Kuyper, C.M.A.
    Purification and enzymatic characterization of three EndoDNase isoenzymes from Physarum polycephalum (1980), J. Biochem., 87, 651-661.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.21.1 less active in phosphate buffer Physarum polycephalum
3.1.21.1 sensitives to repeated freezing and thawing Physarum polycephalum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.1 Ca2+ complete inhibition at 1-10 mM Physarum polycephalum
3.1.21.1 Cu2+ complete inhibition at 1-10 mM Physarum polycephalum
3.1.21.1 EDTA
-
 Physarum polycephalum
3.1.21.1 EGTA activity inhibited Physarum polycephalum
3.1.21.1 Zn2+ complete inhibition at 1-10 mM Physarum polycephalum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.1 Mg2+ 2.0-7.5 mM for DNases A, B or C Physarum polycephalum
3.1.21.1 Mn2+ 0.8-2.5 mM for DNases A, B or C Physarum polycephalum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.1 16000 17000 gel filtration, SDS-PAGE Physarum polycephalum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.1 double-stranded DNA + H2O Physarum polycephalum preference for double-stranded DNA 5'-phosphooligonucleotides + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.1 Physarum polycephalum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.1 to homogeneity by chromatography techniques, isoelectric focusing, gel filtration Physarum polycephalum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.21.1 plasmodium microplasmodium Physarum polycephalum
-

Storage Stability

EC Number Storage Stability Organism
3.1.21.1 -20°C, 10 mM Tris-HCl, pH 7.0, 10 mM KCl, 5 M urea, 6 months Physarum polycephalum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.1 DNA + H2O
-
 Physarum polycephalum 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 double-stranded DNA + H2O preference for double-stranded DNA Physarum polycephalum 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 heat-denatured DNA + H2O
-
 Physarum polycephalum 5'-phosphooligonucleotides + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.21.1 monomer 1 * 16000-17000, four active fractions Physarum polycephalum

Synonyms

EC Number Synonyms Comment Organism
3.1.21.1 DNase A
-
 Physarum polycephalum
3.1.21.1 DNase B
-
 Physarum polycephalum
3.1.21.1 DNase C
-
 Physarum polycephalum
3.1.21.1 EndoDNase
-
 Physarum polycephalum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.1 8.25
-
 for DNase A Physarum polycephalum
3.1.21.1 8.5
-
 for DNase B Physarum polycephalum
3.1.21.1 8.75
-
 for DNase C Physarum polycephalum