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Literature summary extracted from

  • Bellard, M.; Dretzen, G.; Giangrande, A.; Ramain, P.
    Nuclease digestion of transcriptionally active chromatin (1989), Methods Enzymol., 170, 317-346.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.1 Ca2+ addition of Ca2+ stabilizes the enzyme, facilitates second cut in double-stranded DNA Bos taurus
3.1.21.1 Mg2+ presence of Mg2+ alone leads to cutting only one of the two strands Bos taurus
3.1.21.1 Mn2+
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.1 31000
-
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.1 double-stranded DNA + H2O Bos taurus cutting one of the two strands by a nucleophilic attack on the O-3'-P-bond 5'-phosphooligonucleotides + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.1 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.21.1 pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.1 circular plasmid DNA + H2O
-
 Bos taurus 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 DNA + H2O
-
 Bos taurus 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 double-stranded DNA + H2O cutting one of the two strands by a nucleophilic attack on the O-3'-P-bond Bos taurus 5'-phosphooligonucleotides + ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.1 7.8
-
-
 Bos taurus