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Literature summary extracted from

  • Takeshita, H.; Yasuda, T.; Nadano, D.; Iida, R.; Kishi, K.
    Deoxyribonuclease I from rat urine: affinity purification, characterization, and immunochemical studies (1995), J. Biochem., 118, 932-938.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.1 EDTA complete inhibition at 10 mM Rattus sp.
3.1.21.1 EGTA complete inhibition at 10 mM Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.21.1 extracellular secreted into urine Rattus sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.1 Ca2+ 22% stimulation at 10 mM Rattus sp.
3.1.21.1 Co2+ 22% stimulation at 10 mM Rattus sp.
3.1.21.1 Mg2+ 22% stimualtion at 10 mM Rattus sp.
3.1.21.1 Mn2+ 22% stimulation at 10 mM Rattus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.1 32000
-
 SDS-PAGE, gel filtration Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.1 double-stranded DNA + H2O Rattus sp. functionally active during digestion, participation in the repair of damaged DNA 5'-phosphooligonucleotides + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.1 Rattus sp.
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.21.1 glycoprotein contains fucose, mannose, galactose, glucosamine, sialic acid Rattus sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.1 to homogeneity by affinity chromatography Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.21.1 urine
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.21.1 additional information
-
 units defined as absorbance at 260 nm/ml assay solution Rattus sp.
3.1.21.1 additional information
-
 5100 units/mg protein Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.1 DNA + H2O
-
 Rattus sp. 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 double-stranded DNA + H2O functionally active during digestion, participation in the repair of damaged DNA Rattus sp. 5'-phosphooligonucleotides + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.21.1 monomer 1 * 32000, SDS-PAGE, gel filtration Rattus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.1 6.5
-
-
 Rattus sp.