EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.16.1 | analysis | end group and sequential analysis of polynucleotides | Sus scrofa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.16.1 | mitochondrion | - |
Sus scrofa | 5739 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.16.1 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.16.1 | no contamination by other phosphohydrolase activities | Sus scrofa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.16.1 | spleen | - |
Sus scrofa | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.16.1 | 3'-phospho-2'-deoxyribooligonucleotide + H2O | average size 10-12, smallest 3 | Sus scrofa | 2'-deoxyribonucleoside 3'-phosphate | - |
? | |
3.1.16.1 | 4-nitrophenyl phosphate | - |
Sus scrofa | 4-nitrophenol + phosphate | - |
? | |
3.1.16.1 | 5'-hydroxy oligonucleotides + H2O | dependency on type of nucleobase | Sus scrofa | nucleoside 3'-oligonucleotides + nucleoside 3'-phosphates | - |
? | |
3.1.16.1 | bis-(4-nitrophenyl)phosphate + H2O | - |
Sus scrofa | 4-nitrophenyl phosphate + 4-nitrophenol | - |
? | |
3.1.16.1 | DNA + H2O | alkali-denaturated DNA, 25 times lower degradation rate with native DNA | Sus scrofa | deoxyribonucleoside 3'-phosphates | - |
? | |
3.1.16.1 | polyadenylic acid + H2O | - |
Sus scrofa | 5'-hydroxy-adenine oligonucleotides + 3'AMP | - |
? | |
3.1.16.1 | polyinosinic acid | - |
Sus scrofa | 3'-IMP | - |
? | |
3.1.16.1 | polyuridylic acid + H2O | - |
Sus scrofa | 3'-UMP | - |
? | |
3.1.16.1 | RNA core + H2O | RNA core are water undializable 3'-phosphoribooligonucleotides obtained by exhaustive digestion of RNA with pancreatic ribonuclease | Sus scrofa | nucleoside 3'-phosphates | - |
? | |
3.1.16.1 | thymidine 3'-(4-nitrophenyl)phosphate + H2O | very slow hydrolysis | Sus scrofa | thymidine 3'-phosphate + 4-nitrophenol | - |
? | |
3.1.16.1 | tRNA + H2O | from Escherichia coli | Sus scrofa | tRNA fragments + nucleoside 3'-phosphates | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.16.1 | phosphodiesterase II | - |
Sus scrofa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.16.1 | 37 | - |
- |
Sus scrofa |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.16.1 | 45 | 80 | range of thermal inactivation, 50% of activity at 56°C | Sus scrofa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.16.1 | 5.5 | - |
with 0.1 M acetate or succinate buffer | Sus scrofa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.16.1 | 4 | 7 | poor effect of 0.02 M Mg2+ on pH activity curve at 37°C | Sus scrofa |