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Literature summary extracted from

  • Eggleston, A.K.; Kowalczykowski, S.C.
    Biochemical characterization of a mutant recBCD enzyme, the recB2109CD enzyme, which lacks chi-specific, but not non-specific, nuclease activity (1993), J. Mol. Biol., 231, 605-620.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.11.5 analysis no recombination in recB2109CD mutants Escherichia coli
3.1.11.5 analysis possible role in genetic recombination Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.11.5 Mg2+ required, optimal concentration around 3-12 mM Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.11.5 ATP + H2O Escherichia coli
-
 ADP + phosphate
-
 ?
3.1.11.5 double-stranded DNA + H2O Escherichia coli responsible for several steps in genetic recombination process single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O Escherichia coli ATP-dependent exonuclease single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O Escherichia coli ATP-dependent helicase single-stranded DNA fragments
-
 ?
3.1.11.5 single stranded DNA + H2O Escherichia coli ATP-dependent exonuclease 5'-phosphomonoester oligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.5 Escherichia coli
-
 recB2109CD mutant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.11.5 of the abc-modified recBCD protein Escherichia coli
3.1.11.5 of the mutant recB2109CD mutant Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.5 ATP + H2O
-
 Escherichia coli ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O reduced activity in recB2109CD mutant Escherichia coli ADP + phosphate
-
 ?
3.1.11.5 double-stranded DNA no recombination in recB2109CD mutants and abc-modified recBCD mutants Escherichia coli intermediates with single stranded regions
-
 ?
3.1.11.5 double-stranded DNA recB2109CD mutant has no chi nicking activity Escherichia coli intermediates with single stranded regions
-
 ?
3.1.11.5 double-stranded DNA + H2O reduced activity in recB2109CD mutant Escherichia coli single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O responsible for several steps in genetic recombination process Escherichia coli single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP-dependent exonuclease Escherichia coli single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP-dependent helicase Escherichia coli single-stranded DNA fragments
-
 ?
3.1.11.5 single stranded DNA + H2O reduced activity in recB2109CD mutant Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O ATP dependence varies Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O ATP-dependent exonuclease Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?