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Literature summary extracted from

  • Palas, K.M.; Kushner, S.R.
    Biochemical and physical characterization of exonuclease V from Escherichia coli. Comparison of the catalytic activities of the RecBC and RecBCD enzymes (1990), J. Biol. Chem., 265, 3447-3454.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.11.5 RecBC enzyme more instable than the recBCD enzyme Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.11.5 60000
-
 recD protein, sedimentation, SDS-PAGE Escherichia coli
3.1.11.5 270000
-
 gel filtration, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.11.5 ATP + H2O Escherichia coli
-
 ADP + phosphate
-
 ?
3.1.11.5 double-stranded DNA + H2O Escherichia coli ATP-dependent exonuclease single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O Escherichia coli ATP-dependent helicase single-stranded DNA fragments
-
 ?
3.1.11.5 single stranded DNA + H2O Escherichia coli ATP-dependent exonuclease 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O Escherichia coli ATP-dependent endonuclease 5'-phosphomonoester oligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.5 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.11.5 to homogeneity Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.1.11.5 -20°C, purified enzyme RecBC, 1 year, 80% loss of activity Escherichia coli
3.1.11.5 -20°C, purified enzyme RecBCD, 1 year, 10%-20% loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.5 ATP + H2O
-
 Escherichia coli ADP + phosphate
-
 ?
3.1.11.5 ATP + H2O ATPase activity in recBC enzyme Escherichia coli ADP + phosphate
-
 ?
3.1.11.5 double-stranded DNA unwinding activity in the recBC enzyme Escherichia coli intermediates with single stranded regions
-
 ?
3.1.11.5 double-stranded DNA + H2O no exonuclease activity in recD null mutants Escherichia coli single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP-dependent exonuclease Escherichia coli single-stranded DNA fragments
-
 ?
3.1.11.5 double-stranded DNA + H2O ATP-dependent helicase Escherichia coli single-stranded DNA fragments
-
 ?
3.1.11.5 single stranded DNA + H2O no exonuclease activity in recD null mutants Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O endonuclease activity is ATP dependent in recBC enzyme Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O also endonuclease activity on circular DNA Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O ATP dependence varies Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O ATP-dependent exonuclease Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?
3.1.11.5 single stranded DNA + H2O ATP-dependent endonuclease Escherichia coli 5'-phosphomonoester oligonucleotides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.11.5 trimer
-
 Escherichia coli