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Literature summary extracted from

  • Young, D.B.; Rosamond, J.
    Studies on the mechanism of action of the ATP-dependent DNAase from Alcaligenes faecalis (1981), Eur. J. Biochem., 114, 285-292.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.11.5 ATP 0.2 mM and higher concentrations inhibits phosphodiester hydrolysis Alcaligenes faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.11.5 0.09
-
 ATP
-
 Alcaligenes faecalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.11.5 Co2+ stimulates at 1-2 mM Alcaligenes faecalis
3.1.11.5 Mg2+ required, optimal concentration around 3-12 mM Alcaligenes faecalis
3.1.11.5 Mg2+ produces intermediates Alcaligenes faecalis
3.1.11.5 Mn2+ acts processively Alcaligenes faecalis
3.1.11.5 Mn2+ more effective as Mg2+ Alcaligenes faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.11.5 220000
-
 sedimentation, calculated Alcaligenes faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.11.5 ATP + H2O Alcaligenes faecalis
-
 ADP + phosphate
-
 ?
3.1.11.5 single stranded DNA + H2O Alcaligenes faecalis
-
 5'-phosphomonoester oligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.5 Alcaligenes faecalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.11.5 to homogeneity Alcaligenes faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.5 ATP + H2O
-
 Alcaligenes faecalis ADP + phosphate
-
 ?
3.1.11.5 single stranded DNA + H2O
-
 Alcaligenes faecalis 5'-phosphomonoester oligonucleotides
-
 ?