BRENDA - Enzyme Database

Phospholipase A2 from bee venom

Cottrell, R.C.; Methods Enzymol. 71, 698-702 (1981)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.4
Acylating agents
example: fatty acid imidazolides
Apis mellifera
3.1.1.4
EDTA
in small quantities activating: one-tenth the Ca2+-concentration
Apis mellifera
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
EDTA
-
Apis mellifera
3.1.1.4
Maleic anhydride
-
Apis mellifera
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ba2+
can replace Ca2+ but enzyme shows decreased activity
Apis mellifera
3.1.1.4
Ca2+
-
Apis mellifera
3.1.1.4
Mg2+
can replace Ca2+, decreased activity
Apis mellifera
3.1.1.4
Sr2+
can replace Ca2+, decreased activity
Apis mellifera
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
1,2-diacyl-sn-glycero-3-phosphatide + H2O
Apis mellifera
-
?
-
-
?
3.1.1.4
phospholipids + H2O
Apis mellifera
allergenic
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.4
Apis mellifera
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.4
dialysis, column chromatography
Apis mellifera
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
venom
-
Apis mellifera
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
1,2-diacyl-sn-glycero-3-phosphatide + H2O
-
133907
Apis mellifera
1-acyl-sn-glycero-3-phosphatide + fatty acid
-
133907
Apis mellifera
?
3.1.1.4
1,2-diacyl-sn-glycero-3-phosphatide + H2O
-
133907
Apis mellifera
?
-
-
-
?
3.1.1.4
1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O
-
133907
Apis mellifera
1-hexanoyl-sn-glycero-3-phosphocholine + hexanoate
-
133907
Apis mellifera
?
3.1.1.4
1,2-dilauroyl-sn-glycero-3-phosphocholine + H2O
-
133907
Apis mellifera
1-lauroyl-sn-glycero-3-phosphocholine + laureate
-
133907
Apis mellifera
?
3.1.1.4
1,2-dimyristoyl-sn-phosphatidylcholine + H2O
-
133907
Apis mellifera
1-myristoyl-phosphorylcholine + myristic acid
-
133907
Apis mellifera
?
3.1.1.4
1,2-dipalmitoyl-phosphatidylcholine + H2O
-
133907
Apis mellifera
1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
133907
Apis mellifera
?
3.1.1.4
1-oleoyl-2-isolauroyl phosphatidylethanolamine + H2O
-
133907
Apis mellifera
1-oleoyl phosphatidylethanolamine + isolauric acid
-
133907
Apis mellifera
?
3.1.1.4
phosphatidylcholine + H2O
-
133907
Apis mellifera
1-acylglycerophosphocholine + fatty acid
-
133907
Apis mellifera
?
3.1.1.4
phosphatidylglycerol + H2O
-
133907
Apis mellifera
1-acylglycerol + fatty acid
-
-
-
-
3.1.1.4
phospholipids + H2O
allergenic
133907
Apis mellifera
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.4
dimer
dimerization occurs in concentrated aqueous solution, no data concerning MW
Apis mellifera
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.1.1.4
20
-
lyophilized venom PLA2, extremely stable
Apis mellifera
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.4
8
-
substrate: 1,2-dihexanoyl-sn-glycero-3-phosphocholine, in 75% propanol and 25% water
Apis mellifera
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.4
Acylating agents
example: fatty acid imidazolides
Apis mellifera
3.1.1.4
EDTA
in small quantities activating: one-tenth the Ca2+-concentration
Apis mellifera
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
EDTA
-
Apis mellifera
3.1.1.4
Maleic anhydride
-
Apis mellifera
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ba2+
can replace Ca2+ but enzyme shows decreased activity
Apis mellifera
3.1.1.4
Ca2+
-
Apis mellifera
3.1.1.4
Mg2+
can replace Ca2+, decreased activity
Apis mellifera
3.1.1.4
Sr2+
can replace Ca2+, decreased activity
Apis mellifera
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.4
1,2-diacyl-sn-glycero-3-phosphatide + H2O
Apis mellifera
-
?
-
-
?
3.1.1.4
phospholipids + H2O
Apis mellifera
allergenic
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.4
dialysis, column chromatography
Apis mellifera
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
venom
-
Apis mellifera
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
1,2-diacyl-sn-glycero-3-phosphatide + H2O
-
133907
Apis mellifera
1-acyl-sn-glycero-3-phosphatide + fatty acid
-
133907
Apis mellifera
?
3.1.1.4
1,2-diacyl-sn-glycero-3-phosphatide + H2O
-
133907
Apis mellifera
?
-
-
-
?
3.1.1.4
1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O
-
133907
Apis mellifera
1-hexanoyl-sn-glycero-3-phosphocholine + hexanoate
-
133907
Apis mellifera
?
3.1.1.4
1,2-dilauroyl-sn-glycero-3-phosphocholine + H2O
-
133907
Apis mellifera
1-lauroyl-sn-glycero-3-phosphocholine + laureate
-
133907
Apis mellifera
?
3.1.1.4
1,2-dimyristoyl-sn-phosphatidylcholine + H2O
-
133907
Apis mellifera
1-myristoyl-phosphorylcholine + myristic acid
-
133907
Apis mellifera
?
3.1.1.4
1,2-dipalmitoyl-phosphatidylcholine + H2O
-
133907
Apis mellifera
1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
133907
Apis mellifera
?
3.1.1.4
1-oleoyl-2-isolauroyl phosphatidylethanolamine + H2O
-
133907
Apis mellifera
1-oleoyl phosphatidylethanolamine + isolauric acid
-
133907
Apis mellifera
?
3.1.1.4
phosphatidylcholine + H2O
-
133907
Apis mellifera
1-acylglycerophosphocholine + fatty acid
-
133907
Apis mellifera
?
3.1.1.4
phosphatidylglycerol + H2O
-
133907
Apis mellifera
1-acylglycerol + fatty acid
-
-
-
-
3.1.1.4
phospholipids + H2O
allergenic
133907
Apis mellifera
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.4
dimer
dimerization occurs in concentrated aqueous solution, no data concerning MW
Apis mellifera
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.1.1.4
20
-
lyophilized venom PLA2, extremely stable
Apis mellifera
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.4
8
-
substrate: 1,2-dihexanoyl-sn-glycero-3-phosphocholine, in 75% propanol and 25% water
Apis mellifera