BRENDA - Enzyme Database

Phospholipase A2 from Trypanosoma congolense: characterization and haematological properties

Nok, A.J.; Esievo, K.A.N.; Ibrahim, S.; Ukoha, A.I.; Ikediobi, C.O.; Cell Biochem. Funct. 11, 125-130 (1993) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
EDTA
-
Trypanosoma congolense
3.1.1.4
p-hydroxymercuribenzoate
-
Trypanosoma congolense
3.1.1.4
tributyltin oxide
-
Trypanosoma congolense
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.4
1.85
-
dimyristoyl-sn-phosphatidylcholine
-
Trypanosoma congolense
3.1.1.4
2.12
-
dioleolyl-sn-phosphatidylcholine
-
Trypanosoma congolense
3.1.1.4
3.65
-
dimyristoyl-sn-phosphatidylserine
-
Trypanosoma congolense
3.1.1.4
4.6
-
dioleolyl-sn-phosphatidylethanolamine
-
Trypanosoma congolense
3.1.1.4
56
-
dipalmitoyl-sn-phosphatidylserine
-
Trypanosoma congolense
3.1.1.4
58
-
dimyristoyl-sn-phosphatidylinositol
-
Trypanosoma congolense
3.1.1.4
86
-
dipalmitoyl-phosphatidylcholine
-
Trypanosoma congolense
3.1.1.4
98
-
dipalmitoyl-phosphatidylethanolamine
-
Trypanosoma congolense
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Trypanosoma congolense
3.1.1.4
Zn2+
-
Trypanosoma congolense
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
16500
-
1 * 16500 + 1 * 18000, SDS-PAGE
Trypanosoma congolense
3.1.1.4
18000
-
1 * 16500 + 1 * 18000, SDS-PAGE
Trypanosoma congolense
3.1.1.4
34500
-
dimer with a 16500 and a 18000 subunit, SDS-PAGE
Trypanosoma congolense
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.1.1.4
phospholipids + H2O
Trypanosoma congolense
hemolytic activity of basic PLA2
?
-
-
?
3.1.1.4
phospholipids + H2O
Trypanosoma congolense
influence on platelet aggregation
?
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.1.1.4
Trypanosoma congolense
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.1.1.4
ion exchange chromatography
Trypanosoma congolense
Storage Stability
EC Number
Storage Stability
Organism
3.1.1.4
4°C, high stability even at low concentrations, i.e. 50 mg,in 20 mM phosphate buffer pH 6.8 containing 2 mM of 2-mercaptoethanol
Trypanosoma congolense
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.1.1.4
1,2-dimyristoyl-sn-phosphatidylcholine + H2O
-
133884
Trypanosoma congolense
1-myristoyl-phosphorylcholine + myristic acid
-
133884
Trypanosoma congolense
?
3.1.1.4
1,2-dimyristoyl-sn-phosphatidylinositol + H2O
-
133884
Trypanosoma congolense
1-myristoyl-sn-phosphatidylinositol + myristic acid
-
133884
Trypanosoma congolense
?
3.1.1.4
1,2-dimyristoyl-sn-phosphatidylserine + H2O
-
133884
Trypanosoma congolense
1-myristoyl-sn-phosphatidylserine + myristic acid
-
133884
Trypanosoma congolense
?
3.1.1.4
1,2-dioleoyl-sn-phosphatidylcholine + H2O
-
133884
Trypanosoma congolense
1-oleoyl-sn-phosphatidylcholine + oleate
-
133884
Trypanosoma congolense
?
3.1.1.4
1,2-dioleoyl-sn-phosphatidylethanolamine + H2O
-
133884
Trypanosoma congolense
1-oleoyl-sn-phosphatidylethanolamine + oleic acid
-
133884
Trypanosoma congolense
?
3.1.1.4
1,2-dipalmitoyl-phosphatidylcholine + H2O
-
133884
Trypanosoma congolense
1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
133884
Trypanosoma congolense
?
3.1.1.4
1,2-dipalmitoyl-phosphatidylethanolamine + H2O
-
133884
Trypanosoma congolense
1-palmitoyl-phosphatidylethanolamine + palmitic acid
-
133884
Trypanosoma congolense
?
3.1.1.4
1,2-dipalmitoyl-sn-phosphatidylserine + H2O
-
133884
Trypanosoma congolense
1-palmitoyl-sn-phosphatidylserine + palmitic acid
-
133884
Trypanosoma congolense
?
3.1.1.4
1-palmitoyl-2-{6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphocholine + H2O
-
133884
Trypanosoma congolense
1-palmitoyl-sn-glycero-3-phosphocholine + 6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanoic acid
-
133884
Trypanosoma congolense
?
3.1.1.4
dimyristoyl phosphatidylcholine + H2O
-
133884
Trypanosoma congolense
1-myristoyl-sn-glycerophosphocholine + myristic acid
-
-
-
?
3.1.1.4
dimyristoyl phosphatidylinositol + H2O
-
133884
Trypanosoma congolense
1-myristoyl-sn-glycerophosphoinositol + myristic acid
-
-
-
?
3.1.1.4
dimyristoyl phosphatidylserinel + H2O
-
133884
Trypanosoma congolense
1-myristoyl-sn-glycerophosphoserine + myristic acid
-
-
-
?
3.1.1.4
dioleolyl-sn- phosphatidylcholine + H2O
-
133884
Trypanosoma congolense
1-oleolyl-sn-glycerophosphocholine + oleic acid
-
-
-
?
3.1.1.4
dioleolyl-sn- phosphatidylethanolamine + H2O
-
133884
Trypanosoma congolense
1-oleolyl-sn-glycerophosphoethanolamine + oleic acid
-
-
-
?
3.1.1.4
dipalmitoyl- phosphatidylcholine + H2O
-
133884
Trypanosoma congolense
1-palmitoyl-sn-glycerophosphocholine + palmitic acid
-
-
-
?
3.1.1.4
dipalmitoyl- phosphatidylethanolamine + H2O
-
133884
Trypanosoma congolense
1-palmitoyl-sn-glycerophosphoethanolamine + palmitic acid
-
-
-
?
3.1.1.4
phospholipids + H2O
hemolytic activity of basic PLA2
133884
Trypanosoma congolense
?
-
-
-
?
3.1.1.4
phospholipids + H2O
influence on platelet aggregation
133884
Trypanosoma congolense
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.4
dimer
1 * 16500 + 1 * 18000, SDS-PAGE
Trypanosoma congolense
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.4
35
-
-
Trypanosoma congolense
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.4
50
-
enzyme retains 70% of activity after 10 min incubation at 50°C, inactivation above 50°C
Trypanosoma congolense
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.4
6.5
6.8
-
Trypanosoma congolense
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
EDTA
-
Trypanosoma congolense
3.1.1.4
p-hydroxymercuribenzoate
-
Trypanosoma congolense
3.1.1.4
tributyltin oxide
-
Trypanosoma congolense
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.4
1.85
-
dimyristoyl-sn-phosphatidylcholine
-
Trypanosoma congolense
3.1.1.4
2.12
-
dioleolyl-sn-phosphatidylcholine
-
Trypanosoma congolense
3.1.1.4
3.65
-
dimyristoyl-sn-phosphatidylserine
-
Trypanosoma congolense
3.1.1.4
4.6
-
dioleolyl-sn-phosphatidylethanolamine
-
Trypanosoma congolense
3.1.1.4
56
-
dipalmitoyl-sn-phosphatidylserine
-
Trypanosoma congolense
3.1.1.4
58
-
dimyristoyl-sn-phosphatidylinositol
-
Trypanosoma congolense
3.1.1.4
86
-
dipalmitoyl-phosphatidylcholine
-
Trypanosoma congolense
3.1.1.4
98
-
dipalmitoyl-phosphatidylethanolamine
-
Trypanosoma congolense
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.4
Ca2+
-
Trypanosoma congolense
3.1.1.4
Zn2+
-
Trypanosoma congolense
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.4
16500
-
1 * 16500 + 1 * 18000, SDS-PAGE
Trypanosoma congolense
3.1.1.4
18000
-
1 * 16500 + 1 * 18000, SDS-PAGE
Trypanosoma congolense
3.1.1.4
34500
-
dimer with a 16500 and a 18000 subunit, SDS-PAGE
Trypanosoma congolense
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.1.1.4
phospholipids + H2O
Trypanosoma congolense
hemolytic activity of basic PLA2
?
-
-
?
3.1.1.4
phospholipids + H2O
Trypanosoma congolense
influence on platelet aggregation
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.4
ion exchange chromatography
Trypanosoma congolense
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.1.4
4°C, high stability even at low concentrations, i.e. 50 mg,in 20 mM phosphate buffer pH 6.8 containing 2 mM of 2-mercaptoethanol
Trypanosoma congolense
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.1.1.4
1,2-dimyristoyl-sn-phosphatidylcholine + H2O
-
133884
Trypanosoma congolense
1-myristoyl-phosphorylcholine + myristic acid
-
133884
Trypanosoma congolense
?
3.1.1.4
1,2-dimyristoyl-sn-phosphatidylinositol + H2O
-
133884
Trypanosoma congolense
1-myristoyl-sn-phosphatidylinositol + myristic acid
-
133884
Trypanosoma congolense
?
3.1.1.4
1,2-dimyristoyl-sn-phosphatidylserine + H2O
-
133884
Trypanosoma congolense
1-myristoyl-sn-phosphatidylserine + myristic acid
-
133884
Trypanosoma congolense
?
3.1.1.4
1,2-dioleoyl-sn-phosphatidylcholine + H2O
-
133884
Trypanosoma congolense
1-oleoyl-sn-phosphatidylcholine + oleate
-
133884
Trypanosoma congolense
?
3.1.1.4
1,2-dioleoyl-sn-phosphatidylethanolamine + H2O
-
133884
Trypanosoma congolense
1-oleoyl-sn-phosphatidylethanolamine + oleic acid
-
133884
Trypanosoma congolense
?
3.1.1.4
1,2-dipalmitoyl-phosphatidylcholine + H2O
-
133884
Trypanosoma congolense
1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
133884
Trypanosoma congolense
?
3.1.1.4
1,2-dipalmitoyl-phosphatidylethanolamine + H2O
-
133884
Trypanosoma congolense
1-palmitoyl-phosphatidylethanolamine + palmitic acid
-
133884
Trypanosoma congolense
?
3.1.1.4
1,2-dipalmitoyl-sn-phosphatidylserine + H2O
-
133884
Trypanosoma congolense
1-palmitoyl-sn-phosphatidylserine + palmitic acid
-
133884
Trypanosoma congolense
?
3.1.1.4
1-palmitoyl-2-{6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphocholine + H2O
-
133884
Trypanosoma congolense
1-palmitoyl-sn-glycero-3-phosphocholine + 6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanoic acid
-
133884
Trypanosoma congolense
?
3.1.1.4
dimyristoyl phosphatidylcholine + H2O
-
133884
Trypanosoma congolense
1-myristoyl-sn-glycerophosphocholine + myristic acid
-
-
-
?
3.1.1.4
dimyristoyl phosphatidylinositol + H2O
-
133884
Trypanosoma congolense
1-myristoyl-sn-glycerophosphoinositol + myristic acid
-
-
-
?
3.1.1.4
dimyristoyl phosphatidylserinel + H2O
-
133884
Trypanosoma congolense
1-myristoyl-sn-glycerophosphoserine + myristic acid
-
-
-
?
3.1.1.4
dioleolyl-sn- phosphatidylcholine + H2O
-
133884
Trypanosoma congolense
1-oleolyl-sn-glycerophosphocholine + oleic acid
-
-
-
?
3.1.1.4
dioleolyl-sn- phosphatidylethanolamine + H2O
-
133884
Trypanosoma congolense
1-oleolyl-sn-glycerophosphoethanolamine + oleic acid
-
-
-
?
3.1.1.4
dipalmitoyl- phosphatidylcholine + H2O
-
133884
Trypanosoma congolense
1-palmitoyl-sn-glycerophosphocholine + palmitic acid
-
-
-
?
3.1.1.4
dipalmitoyl- phosphatidylethanolamine + H2O
-
133884
Trypanosoma congolense
1-palmitoyl-sn-glycerophosphoethanolamine + palmitic acid
-
-
-
?
3.1.1.4
phospholipids + H2O
hemolytic activity of basic PLA2
133884
Trypanosoma congolense
?
-
-
-
?
3.1.1.4
phospholipids + H2O
influence on platelet aggregation
133884
Trypanosoma congolense
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.4
dimer
1 * 16500 + 1 * 18000, SDS-PAGE
Trypanosoma congolense
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.4
35
-
-
Trypanosoma congolense
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.4
50
-
enzyme retains 70% of activity after 10 min incubation at 50°C, inactivation above 50°C
Trypanosoma congolense
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.4
6.5
6.8
-
Trypanosoma congolense