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Literature summary extracted from

  • Rudd, E.A.; Mizuno, N.K.; Brockman, H.L.
    Isolation of two forms of carboxylester lipase (cholesterol esterase) from porcine pancreas (1987), Biochim. Biophys. Acta, 918, 106-114.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.13 74000
-
 native and denaturating PAGE, low-molecular weight form Sus scrofa
3.1.1.13 83000
-
 native and denaturating PAGE, high-molecular weight form Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.13 cholesteryl esters + H2O Sus scrofa absoprtion of free cholesterol cholesterol + fatty acid
-
 r
3.1.1.13 fat-soluble vitamin esters + H2O Sus scrofa
-
 vitamin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.13 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.13 to homogeneity by chromatography techniques Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.13 pancreas
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.13 12.2
-
 for cholesteryl oleate Sus scrofa
3.1.1.13 19.5
-
 for p-nitrophenyl acetate Sus scrofa
3.1.1.13 53.3
-
 for high-molecular weight form Sus scrofa
3.1.1.13 60.5
-
 for low-molecular weight form Sus scrofa
3.1.1.13 333
-
 for tributyrin Sus scrofa

Storage Stability

EC Number Storage Stability Organism
3.1.1.13 -70°C, 6 months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.13 cholesteryl esters + H2O absoprtion of free cholesterol Sus scrofa cholesterol + fatty acid
-
 r
3.1.1.13 cholesteryl oleate + H2O
-
 Sus scrofa cholesterol + oleic acid
-
 r
3.1.1.13 fat-soluble vitamin esters + H2O
-
 Sus scrofa vitamin + ?
-
 ?
3.1.1.13 p-nitrophenyl acetate + H2O
-
 Sus scrofa p-nitrophenol + acetic acid
-
 ?
3.1.1.13 tributyrin + H2O
-
 Sus scrofa dibutyrylglycerol + butanoic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.13 dimer 2 * 83000, native and denaturating PAGE Sus scrofa
3.1.1.13 monomer 1 * 74000, native and denaturating PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
3.1.1.13 carboxylester lipase
-
 Sus scrofa
3.1.1.13 carboxylesterase
-
 Sus scrofa