BRENDA - Enzyme Database

Hydrolysis of cholesteryl oleate liquid crystals by hormon-sensitive lipase

Tsujita,T.; Okuda, H.; J. Biochem. 113, 264-269 (1993)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.13
cAMP-dependent protein kinase type II
increasing activity on triolein in presence of Mg-ATP2-
Bos taurus
3.1.1.13
phosphatidylcholine
slightly activating
Bos taurus
3.1.1.13
phosphatidylethanolamine
slightly activating
Bos taurus
3.1.1.79
phosphatidylcholine
0.075 mM, slight activation upon incorporation into cholestryl oleate liquid crystals
Bos taurus
3.1.1.79
phosphatidylethanolamine
0.075 mM, approx. 6fold activation upon incorporation into cholestryl oleate liquid crystals
Bos taurus
General Stability
EC Number
General Stability
Organism
3.1.1.13
less stable in Tris-HCl buffer than in potassium phosphate buffer
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.13
phosphatidic acid
slight inhibition
Bos taurus
3.1.1.13
phosphatidylcholine
slightly inhibitory at higher concentrations
Bos taurus
3.1.1.13
phosphatidylethanolamine
-
Bos taurus
3.1.1.13
phosphatidylserine
complete inhibition at 0.05 mM for cholesteryl oleate hydrolysis, for triolein hydrolysis 65% inhibition, p-nitrophenyl butyrate hydrolysis not affected
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.13
0.011
-
cholesteryl oleate
in cholesteryl oleate vesicles
Bos taurus
3.1.1.13
0.012
-
cholesteryl oleate
in mixed emulsion of cholesteryl oleate, phosphatidylcholine and taurocholic acid
Bos taurus
3.1.1.13
0.099
-
cholesteryl oleate
in liquid crystals
Bos taurus
3.1.1.79
0.011
-
cholesteyl oleate
cholesteryl oleate vesicles, 37°C, pH 6.8
Bos taurus
3.1.1.79
0.012
-
cholesteyl oleate
cholesteryl oleate emulsion, 37°C, pH 6.8
Bos taurus
3.1.1.79
0.099
-
cholesteyl oleate
liquid crystals of cholesteryl oleate, 37°C, pH 6.8
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.13
cholesteryl esters + H2O
Bos taurus
supplies free cholesterol as a precursor for steroidogenesis in steroid-producing tissues
cholesterol + fatty acid
-
Bos taurus
-
3.1.1.13
cholesteryl esters + H2O
Bos taurus
involvement in arteriosclerosis
cholesterol + fatty acid
-
Bos taurus
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.13
Bos taurus
-
-
-
3.1.1.79
Bos taurus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.13
adipose tissue
-
Bos taurus
-
3.1.1.79
adipose tissue
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.13
0.153
-
in liquid crystals of cholesteryl oleate
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.13
cholesteryl esters + H2O
supplies free cholesterol as a precursor for steroidogenesis in steroid-producing tissues
133829
Bos taurus
cholesterol + fatty acid
-
133829
Bos taurus
-
3.1.1.13
cholesteryl esters + H2O
involvement in arteriosclerosis
133829
Bos taurus
cholesterol + fatty acid
-
133829
Bos taurus
-
3.1.1.13
cholesteryl oleate + H2O
-
133829
Bos taurus
cholesterol + oleic acid
-
133829
Bos taurus
?
3.1.1.13
p-nitrophenyl butyrate + H2O
-
133829
Bos taurus
p-nitrophenol + butanoic acid
-
133829
Bos taurus
?
3.1.1.13
triolein + H2O
-
133829
Bos taurus
dioleoylglycerol + oleic acid
-
133829
Bos taurus
?
3.1.1.79
cholesteryl oleate + H2O
-
133829
Bos taurus
cholesterol + oleate
-
-
-
?
3.1.1.79
p-nitrophenyl butyrate + H2O
-
133829
Bos taurus
p-nitrophenol + butanoate
-
-
-
?
3.1.1.79
triolein + H2O
-
133829
Bos taurus
dioleoylglycerol + oleate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.13
6.8
-
measured in liquid crystals of cholesteryl oleate
Bos taurus
3.1.1.79
6.8
-
-
Bos taurus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.79
5
9
approx. 50% of maximal activity at pH 5.5 and pH 8.5, respectively
Bos taurus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
3.1.1.13
MgATP2-
increasing activity with addition of cAMP-dependend protein kinase
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.13
cAMP-dependent protein kinase type II
increasing activity on triolein in presence of Mg-ATP2-
Bos taurus
3.1.1.13
phosphatidylcholine
slightly activating
Bos taurus
3.1.1.13
phosphatidylethanolamine
slightly activating
Bos taurus
3.1.1.79
phosphatidylcholine
0.075 mM, slight activation upon incorporation into cholestryl oleate liquid crystals
Bos taurus
3.1.1.79
phosphatidylethanolamine
0.075 mM, approx. 6fold activation upon incorporation into cholestryl oleate liquid crystals
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
3.1.1.13
MgATP2-
increasing activity with addition of cAMP-dependend protein kinase
Bos taurus
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.1.13
less stable in Tris-HCl buffer than in potassium phosphate buffer
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.13
phosphatidic acid
slight inhibition
Bos taurus
3.1.1.13
phosphatidylcholine
slightly inhibitory at higher concentrations
Bos taurus
3.1.1.13
phosphatidylethanolamine
-
Bos taurus
3.1.1.13
phosphatidylserine
complete inhibition at 0.05 mM for cholesteryl oleate hydrolysis, for triolein hydrolysis 65% inhibition, p-nitrophenyl butyrate hydrolysis not affected
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.13
0.011
-
cholesteryl oleate
in cholesteryl oleate vesicles
Bos taurus
3.1.1.13
0.012
-
cholesteryl oleate
in mixed emulsion of cholesteryl oleate, phosphatidylcholine and taurocholic acid
Bos taurus
3.1.1.13
0.099
-
cholesteryl oleate
in liquid crystals
Bos taurus
3.1.1.79
0.011
-
cholesteyl oleate
cholesteryl oleate vesicles, 37°C, pH 6.8
Bos taurus
3.1.1.79
0.012
-
cholesteyl oleate
cholesteryl oleate emulsion, 37°C, pH 6.8
Bos taurus
3.1.1.79
0.099
-
cholesteyl oleate
liquid crystals of cholesteryl oleate, 37°C, pH 6.8
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.13
cholesteryl esters + H2O
Bos taurus
supplies free cholesterol as a precursor for steroidogenesis in steroid-producing tissues
cholesterol + fatty acid
-
Bos taurus
-
3.1.1.13
cholesteryl esters + H2O
Bos taurus
involvement in arteriosclerosis
cholesterol + fatty acid
-
Bos taurus
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.13
adipose tissue
-
Bos taurus
-
3.1.1.79
adipose tissue
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.13
0.153
-
in liquid crystals of cholesteryl oleate
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.13
cholesteryl esters + H2O
supplies free cholesterol as a precursor for steroidogenesis in steroid-producing tissues
133829
Bos taurus
cholesterol + fatty acid
-
133829
Bos taurus
-
3.1.1.13
cholesteryl esters + H2O
involvement in arteriosclerosis
133829
Bos taurus
cholesterol + fatty acid
-
133829
Bos taurus
-
3.1.1.13
cholesteryl oleate + H2O
-
133829
Bos taurus
cholesterol + oleic acid
-
133829
Bos taurus
?
3.1.1.13
p-nitrophenyl butyrate + H2O
-
133829
Bos taurus
p-nitrophenol + butanoic acid
-
133829
Bos taurus
?
3.1.1.13
triolein + H2O
-
133829
Bos taurus
dioleoylglycerol + oleic acid
-
133829
Bos taurus
?
3.1.1.79
cholesteryl oleate + H2O
-
133829
Bos taurus
cholesterol + oleate
-
-
-
?
3.1.1.79
p-nitrophenyl butyrate + H2O
-
133829
Bos taurus
p-nitrophenol + butanoate
-
-
-
?
3.1.1.79
triolein + H2O
-
133829
Bos taurus
dioleoylglycerol + oleate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.13
6.8
-
measured in liquid crystals of cholesteryl oleate
Bos taurus
3.1.1.79
6.8
-
-
Bos taurus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.79
5
9
approx. 50% of maximal activity at pH 5.5 and pH 8.5, respectively
Bos taurus