Any feedback?
Literature summary extracted from
- Hydrolysis of cholesteryl oleate liquid crystals by hormon-sensitive lipase (1993), J. Biochem., 113, 264-269.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.13 | cAMP-dependent protein kinase type II | increasing activity on triolein in presence of Mg-ATP2- | Bos taurus | |
| 3.1.1.13 | phosphatidylcholine | slightly activating | Bos taurus |  |
| 3.1.1.13 | phosphatidylethanolamine | slightly activating | Bos taurus | |
| 3.1.1.79 | phosphatidylcholine | 0.075 mM, slight activation upon incorporation into cholestryl oleate liquid crystals | Bos taurus | |
| 3.1.1.79 | phosphatidylethanolamine | 0.075 mM, approx. 6fold activation upon incorporation into cholestryl oleate liquid crystals | Bos taurus | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.1.13 | less stable in Tris-HCl buffer than in potassium phosphate buffer | Bos taurus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.13 | phosphatidic acid | slight inhibition | Bos taurus | |
| 3.1.1.13 | phosphatidylcholine | slightly inhibitory at higher concentrations | Bos taurus | |
| 3.1.1.13 | phosphatidylethanolamine | - |
Bos taurus | |
| 3.1.1.13 | phosphatidylserine | complete inhibition at 0.05 mM for cholesteryl oleate hydrolysis, for triolein hydrolysis 65% inhibition, p-nitrophenyl butyrate hydrolysis not affected | Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.13 | 0.011 | - |
cholesteryl oleate | in cholesteryl oleate vesicles | Bos taurus | |
| 3.1.1.13 | 0.012 | - |
cholesteryl oleate | in mixed emulsion of cholesteryl oleate, phosphatidylcholine and taurocholic acid | Bos taurus | |
| 3.1.1.13 | 0.099 | - |
cholesteryl oleate | in liquid crystals | Bos taurus | |
| 3.1.1.79 | 0.011 | - |
cholesteryl oleate | cholesteryl oleate vesicles, 37°C, pH 6.8 | Bos taurus | |
| 3.1.1.79 | 0.012 | - |
cholesteryl oleate | cholesteryl oleate emulsion, 37°C, pH 6.8 | Bos taurus | |
| 3.1.1.79 | 0.099 | - |
cholesteryl oleate | liquid crystals of cholesteryl oleate, 37°C, pH 6.8 | Bos taurus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.13 | cholesteryl esters + H2O | Bos taurus | supplies free cholesterol as a precursor for steroidogenesis in steroid-producing tissues | cholesterol + fatty acid | - |
? | |
| 3.1.1.13 | cholesteryl esters + H2O | Bos taurus | involvement in arteriosclerosis | cholesterol + fatty acid | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.13 | Bos taurus | - |
- |
- |
| 3.1.1.79 | Bos taurus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.13 | adipose tissue | - |
Bos taurus | - |
| 3.1.1.79 | adipose tissue | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.13 | 0.153 | - |
in liquid crystals of cholesteryl oleate | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.13 | cholesteryl esters + H2O | supplies free cholesterol as a precursor for steroidogenesis in steroid-producing tissues | Bos taurus | cholesterol + fatty acid | - |
? | |
| 3.1.1.13 | cholesteryl esters + H2O | involvement in arteriosclerosis | Bos taurus | cholesterol + fatty acid | - |
? | |
| 3.1.1.13 | cholesteryl oleate + H2O | - |
Bos taurus | cholesterol + oleic acid | - |
? | |
| 3.1.1.13 | p-nitrophenyl butyrate + H2O | - |
Bos taurus | p-nitrophenol + butanoic acid | - |
? | |
| 3.1.1.13 | triolein + H2O | - |
Bos taurus | dioleoylglycerol + oleic acid | - |
? | |
| 3.1.1.79 | cholesteryl oleate + H2O | - |
Bos taurus | cholesterol + oleate | - |
? | |
| 3.1.1.79 | p-nitrophenyl butyrate + H2O | - |
Bos taurus | p-nitrophenol + butanoate | - |
? | |
| 3.1.1.79 | triolein + H2O | - |
Bos taurus | dioleoylglycerol + oleate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.13 | hormone-sensitive lipase | - |
Bos taurus |
| 3.1.1.13 | HSL | - |
Bos taurus |
| 3.1.1.79 | HSL | - |
Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.13 | 6.8 | - |
measured in liquid crystals of cholesteryl oleate | Bos taurus |
| 3.1.1.79 | 6.8 | - |
- |
Bos taurus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.79 | 5 | 9 | approx. 50% of maximal activity at pH 5.5 and pH 8.5, respectively | Bos taurus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.13 | MgATP2- | increasing activity with addition of cAMP-dependend protein kinase | Bos taurus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
