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Literature summary extracted from

  • Dando, P.R.
    Strombine [N-(carboxymethyl)-D-alanine]dehydrogenase and alanopine [meso-N-(1-carboxyethyl)-alanine]dehydrogenase from the mussel Mytilus edulis L. (1981), Biochem. Soc. Trans., 9, 297-298.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.17 0.03
-
 NAD+
-
 Mytilus edulis
1.5.1.17 1
-
 alanopine
-
 Mytilus edulis
1.5.1.17 10
-
 L-Ala
-
 Mytilus edulis
1.5.1.17 50
-
 Gly
-
 Mytilus edulis
1.5.1.22 40
-
 L-Ala Gly Mytilus edulis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.17 cytosol
-
 Mytilus edulis 5829
-
1.5.1.22 cytosol
-
 Mytilus edulis 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.17 38500
-
 gel filtration Mytilus edulis
1.5.1.22 38200
-
 gel filtration Mytilus edulis

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.17 Mytilus edulis
-
-
-
1.5.1.22 Mytilus edulis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.22 partial Mytilus edulis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.17 adductor
-
 Mytilus edulis
-
1.5.1.22 adductor
-
 Mytilus edulis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.17 Gly + pyruvate + NADH at 9% of the activity with L-Ala Mytilus edulis Strombine + NAD+ + H2O
-
 ?
1.5.1.17 L-Ala + 2-oxobutanoate + NADH at 14% of the activity with pyruvate Mytilus edulis ?
-
 ?
1.5.1.17 L-Ala + oxaloacetate + NADH as effective as pyruvate Mytilus edulis ?
-
 ?
1.5.1.17 L-Ala + pyruvate + NADH r Mytilus edulis 2,2'-Iminodipropanoate + NAD+ + H2O
-
 ?
1.5.1.17 L-Cys + pyruvate + NADH at 110% of the activity with L-Ala Mytilus edulis ?
-
 ?
1.5.1.17 L-Ser + pyruvate + NADH at 67% of the activity with L-Ala Mytilus edulis ?
-
 ?
1.5.1.22 Gly + pyruvate + NADH r Mytilus edulis D-Strombine + NAD+ + H2O
-
 ?
1.5.1.22 L-Ala + pyruvate + NADH r Mytilus edulis meso-Alanopine + NAD+ + H2O
-
 ?
1.5.1.22 L-Ala + pyruvate + NADH in the reverse reaction: 81% of the activity with meso-alanopine compared to strombine Mytilus edulis meso-Alanopine + NAD+ + H2O
-
 ?
1.5.1.22 L-Met + pyruvate + NADH 3.7% of the activity with Gly Mytilus edulis ?
-
 ?
1.5.1.22 L-Ser + pyruvate + NADH 31% of the activity with Gly Mytilus edulis ?
-
 ?
1.5.1.22 L-Thr + pyruvate + NADH 3.7% of the activity with Gly Mytilus edulis ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.17 7
-
 with Ala and pyruvate as substrates Mytilus edulis
1.5.1.17 9
-
 with meso-alanopine as substrate Mytilus edulis
1.5.1.22 7.2
-
 with Gly and pyruvate as substrates Mytilus edulis
1.5.1.22 9
-
 with strombine as substrate Mytilus edulis

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.17 NAD+ no activity with NADP+ Mytilus edulis
1.5.1.17 NADH no activity with NADPH Mytilus edulis
1.5.1.22 NAD+ no activity with NADP+ Mytilus edulis
1.5.1.22 NADH no activity with NADPH Mytilus edulis