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Literature summary extracted from

  • Nitta, Y.; Yasuda, Y.; Tochikubo, K.; Hachisuka, Y.
    L-Amino acid dehydrogenases in Bacillus subtilis spores (1974), J. Bacteriol., 117, 588-592.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.1 Zn2+
-
 Bacillus subtilis
1.4.1.5 Zn2+
-
 Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 0.36
-
 NAD+
-
 Bacillus subtilis
1.4.1.1 5.4
-
 L-Ala
-
 Bacillus subtilis
1.4.1.5 0.16
-
 NAD+ (+ L-isoleucine) Bacillus subtilis
1.4.1.5 2.2
-
 L-isoleucine
-
 Bacillus subtilis
1.4.1.5 25
-
 L-valine
-
 Bacillus subtilis
1.4.1.5 33
-
 NAD+ (+ L-valine) Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.1 280000
-
 gel filtration Bacillus subtilis
1.4.1.5 290000
-
 gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.5 L-Amino acid + H2O + NAD+ Bacillus subtilis
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Bacillus subtilis
-
-
-
1.4.1.5 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1
-
 Bacillus subtilis
1.4.1.5 partial Bacillus subtilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.1.1 spore resting Bacillus subtilis
-
1.4.1.5 spore resting Bacillus subtilis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 L-Ala + H2O + NAD+
-
 Bacillus subtilis pyruvate + NH3 + NADH
-
 r
1.4.1.5 L-Amino acid + H2O + NAD+ aliphatic amino acids, e.g. L-valine, L-leucine, L-isoleucine Bacillus subtilis 2-Oxo acid + NH3 + NADH
-
 ?
1.4.1.5 L-Amino acid + H2O + NAD+
-
 Bacillus subtilis ?
-
 ?
1.4.1.5 L-isoleucine + H2O + NAD+
-
 Bacillus subtilis 3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 r
1.4.1.5 L-valine + H2O + NAD+
-
 Bacillus subtilis 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.1 hexamer
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.5 37
-
 assay at Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.1 20
-
 10 min, stable Bacillus subtilis
1.4.1.5 20
-
 10 min, 25-35% loss of activity Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.1 10.1
-
-
 Bacillus subtilis
1.4.1.5 10.7
-
 L-valine, L-isoleucine Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
 Bacillus subtilis
1.4.1.1 NADH
-
 Bacillus subtilis
1.4.1.5 NAD+ cannot be replaced by NADP+ Bacillus subtilis