EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.99.18 | Acriflavine | - |
Pseudomonas sp. | |
1.3.99.18 | CoCl2 | - |
Pseudomonas sp. | |
1.3.99.18 | Cu2+ | - |
Pseudomonas sp. | |
1.3.99.18 | KCN | 74% reactivation with S2- | Pseudomonas sp. | |
1.3.99.18 | methanol | - |
Pseudomonas sp. | |
1.3.99.18 | Mn2+ | - |
Pseudomonas sp. | |
1.3.99.18 | NaAsO2 | - |
Pseudomonas sp. | |
1.3.99.18 | NiCl2 | - |
Pseudomonas sp. | |
1.3.99.18 | p-hydroxymercuribenzoate | - |
Pseudomonas sp. | |
1.3.99.18 | Quinacrine | - |
Pseudomonas sp. | |
1.3.99.18 | ZnSO4 | - |
Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.99.18 | 0.023 | - |
Quinaldate | with 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride as cosubstrate | Pseudomonas sp. | |
1.3.99.18 | 1.6 | - |
2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride | with quinaldate as cosubstrate | Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.3.99.18 | Iron | contains 7.6 g-atom of iron per mol of enzyme and 7.5 g-atom of acid-labile sulfur per mol of enzyme | Pseudomonas sp. | |
1.3.99.18 | Molybdenum | enzyme contains 1.6 g-atom per mol of enzyme | Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.99.18 | 20000 | - |
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE | Pseudomonas sp. |
1.3.99.18 | 32000 | - |
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE | Pseudomonas sp. |
1.3.99.18 | 90000 | - |
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE | Pseudomonas sp. |
1.3.99.18 | 300000 | - |
gel filtration | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.99.18 | Pseudomonas sp. | - |
AK-2 | - |
1.3.99.18 | Pseudomonas sp. AK-2 | - |
AK-2 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.99.18 | - |
Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.99.18 | 7.82 | - |
- |
Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.99.18 | quinaldate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O | - |
Pseudomonas sp. | kynurenate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride | - |
r | |
1.3.99.18 | quinaldate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O | - |
Pseudomonas sp. AK-2 | kynurenate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride | - |
r | |
1.3.99.18 | quinaldate + acceptor | acceptors: 1,4-benzoquinone, 1,2-naphthoquinone, nitro blue tetrazolium chloride, 2,6-dichlorophenol indophenol, thionine, menadione, 2,4,6-trinitrobenzo sulfonic acid | Pseudomonas sp. | kynurenate + reduced acceptor | i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate | ? | |
1.3.99.18 | quinaldate + acceptor | i.e. quinoline 2-carboxylic acid | Pseudomonas sp. | kynurenate + reduced acceptor | i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate | ? | |
1.3.99.18 | quinaldate + acceptor | acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride | Pseudomonas sp. | kynurenate + reduced acceptor | i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate | ? | |
1.3.99.18 | quinaldate + acceptor | acceptors: 1,4-benzoquinone, 1,2-naphthoquinone, nitro blue tetrazolium chloride, 2,6-dichlorophenol indophenol, thionine, menadione, 2,4,6-trinitrobenzo sulfonic acid | Pseudomonas sp. AK-2 | kynurenate + reduced acceptor | i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate | ? | |
1.3.99.18 | quinaldate + acceptor | i.e. quinoline 2-carboxylic acid | Pseudomonas sp. AK-2 | kynurenate + reduced acceptor | i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate | ? | |
1.3.99.18 | quinaldate + acceptor | acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride | Pseudomonas sp. AK-2 | kynurenate + reduced acceptor | i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate | ? | |
1.3.99.18 | quinoline 8-carboxylic acid + reduced acceptor | - |
Pseudomonas sp. | ? | - |
? | |
1.3.99.18 | quinoline 8-carboxylic acid + reduced acceptor | - |
Pseudomonas sp. AK-2 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.99.18 | hexamer | 2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE | Pseudomonas sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.99.18 | 60 | - |
above 60°C | Pseudomonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.99.18 | 75 | - |
5 min, 32% loss of activity | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.99.18 | 8 | - |
- |
Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.99.18 | FAD | enzyme contains 1.8 mol of FAD per mol of enzyme | Pseudomonas sp. | |
1.3.99.18 | Pterin molybdenum cofactor | molybdenum is probably associated with molybdopterin cytosine dinucleotide as organic part of the cofactor, 2 mol of molybdopterin cytosine dinucleotide per mol of enzyme | Pseudomonas sp. |