Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bugg, T.D.H.; Dutka-Malen, S.; Athur, M.; Courvalin, P.; Walsh, C.T.
    Identification of vancomycin resistance protein VanA as a D-alanine:D-alanine ligase of altered substrate specificity (1991), Biochemistry, 30, 2017-2021.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.4 D-cycloserine
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.4 additional information
-
 additional information
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.4 ATP + Ala + Ala Escherichia coli a possible cellular role of VanA is to synthesize a modified cell-wall component, with D-Ala-D-Met or D-Ala-Phe instead of D-Ala-D-Ala, which is subsequently not recognized by vancomycin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.4 Escherichia coli
-
 containing recombinant plasmid pAT214, which expressed the VanA protein
-
6.3.2.4 Escherichia coli JM105
-
 containing recombinant plasmid pAT214, which expressed the VanA protein
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.4 Van A Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.4 7.9
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.4 ATP + Ala + Ala a possible cellular role of VanA is to synthesize a modified cell-wall component, with D-Ala-D-Met or D-Ala-Phe instead of D-Ala-D-Ala, which is subsequently not recognized by vancomycin Escherichia coli ?
-
 ?
6.3.2.4 ATP + D-Ala + D-2-aminopentanoate
-
 Escherichia coli ADP + phosphate + D-Ala-D-2-aminopentanoate
-
 ?
6.3.2.4 ATP + D-Ala + D-2-aminopentanoate
-
 Escherichia coli JM105 ADP + phosphate + D-Ala-D-2-aminopentanoate
-
 ?
6.3.2.4 ATP + D-Ala + D-Ala
-
 Escherichia coli ADP + phosphate + D-Ala-D-Ala
-
 ?
6.3.2.4 ATP + D-Ala + D-Ala
-
 Escherichia coli JM105 ADP + phosphate + D-Ala-D-Ala
-
 ?
6.3.2.4 ATP + D-Ala + D-Met
-
 Escherichia coli ADP + phosphate + D-Ala-D-Met
-
 ?
6.3.2.4 ATP + D-Ala + D-Met
-
 Escherichia coli JM105 ADP + phosphate + D-Ala-D-Met
-
 ?
6.3.2.4 ATP + D-Ala + D-norleucine
-
 Escherichia coli ADP + phosphate + D-Ala-D-norleucine
-
 ?
6.3.2.4 ATP + D-Ala + D-norleucine
-
 Escherichia coli JM105 ADP + phosphate + D-Ala-D-norleucine
-
 ?
6.3.2.4 ATP + D-Ala + D-Phe
-
 Escherichia coli ADP + phosphate + D-Ala-D-Phe
-
 ?
6.3.2.4 ATP + D-Ala + D-Phe
-
 Escherichia coli JM105 ADP + phosphate + D-Ala-D-Phe
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.4 4.92
-
 D-Ala
-
 Escherichia coli