Literature summary extracted from
Tanaka, T.; Yamaguchi, H.; Kato, H.; Nishioka, T.; Katsube, Y.; Oda, J.
Flexibility impaired by mutations related the multifunctional roles of the loop in glutathione synthetase (1993), Biochemistry, 32, 12398-12404.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
6.3.2.3 |
mutant enzymes replaced with Val at the basal position of the flexible loop (P227V, G240V, and P227V/G240V) are identical with wild-type enzyme in their crystal structures, except the loop region |
Escherichia coli |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
6.3.2.3 |
G240V |
mutant enzymes replaced with Val at the basal position of the flexible loop (P227V, G240V, and P227V/G240V) are identical with wild-type enzyme in their crystal structures, except the loop region |
Escherichia coli |
6.3.2.3 |
P227V |
mutant enzymes replaced with Val at the basal position of the flexible loop (P227V, G240V, and P227V/G240V) are identical with wild-type enzyme in their crystal structures, except the loop region |
Escherichia coli |
6.3.2.3 |
P227V/G240V |
mutant enzymes replaced with Val at the basal position of the flexible loop (P227V, G240V, and P227V/G240V) are identical with wild-type enzyme in their crystal structures, except the loop region |
Escherichia coli |
General Stability
EC Number |
General Stability |
Organism |
---|
6.3.2.3 |
the remaining activity after arginyl-endopeptidase treatment is higher in the presence of ATP and/or gamma-Glu-Cys than in their absence |
Escherichia coli |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
6.3.2.3 |
additional information |
- |
additional information |
Km-values for Gly and gamma-Glu-Cys are sensitive to the mutation and drastically increased |
Escherichia coli |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
6.3.2.3 |
Escherichia coli |
- |
wild-type and mutant enzymes |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
6.3.2.3 |
ATP + gamma-Glu-L-Cys + Gly |
- |
Escherichia coli |
ADP + phosphate + glutathione |
- |
? |
|
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
6.3.2.3 |
additional information |
- |
additional information |
turnover number is fatally reduced in the P227V/G240V mutant |
Escherichia coli |
|