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Literature summary extracted from

  • Bertram, P.A.; Schmitz, R.A.; Linder, D.; Thauer, R.K.
    Tungstate can substitute for molybdate in sustaining growth of Methanobacterium thermoautotrophicum. Identification and characterization of a tungsten isoenzyme of formylmethanofuran dehydrogenase (1994), Arch. Microbiol., 161, 220-228.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.12 0.03
-
 N-formylmethanofuran
-
 Methanothermobacter thermautotrophicus
1.2.7.12 0.8
-
 methylviologen
-
 Methanothermobacter thermautotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.12 Iron contains 8 mol of non-heme iron and 8 mol of acid-labile sulfur per mol of enzyme Methanothermobacter thermautotrophicus
1.2.7.12 Tungsten tungsten-containing isoenzyme: contains 0.4 mol of tungsten per mol of enzyme Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.12 15000
-
 1 * 65000 + 1 * 53000 + 1 * 31000 + 1 * 15000, SDS-PAGE Methanothermobacter thermautotrophicus
1.2.7.12 31000
-
 1 * 65000 + 1 * 53000 + 1 * 31000 + 1 * 15000, SDS-PAGE Methanothermobacter thermautotrophicus
1.2.7.12 53000
-
 1 * 65000 + 1 * 53000 + 1 * 31000 + 1 * 15000, SDS-PAGE Methanothermobacter thermautotrophicus
1.2.7.12 65000
-
 1 * 65000 + 1 * 53000 + 1 * 31000 + 1 * 15000, SDS-PAGE Methanothermobacter thermautotrophicus
1.2.7.12 160000
-
 gel filtration Methanothermobacter thermautotrophicus

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.12 Methanothermobacter thermautotrophicus
-
-
-
1.2.7.12 Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.12 tungsten-containing isoenzyme Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.12 additional information
-
-
 Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.12 formylmethanofuran + oxidized methylviologen + H2O
-
 Methanothermobacter thermautotrophicus methanofuran + reduced methylviologen + CO2
-
 ?
1.2.7.12 formylmethanofuran + oxidized methylviologen + H2O
-
 Methanothermobacter thermautotrophicus Marburg / DSM 2133 methanofuran + reduced methylviologen + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.12 tetramer 1 * 65000 + 1 * 53000 + 1 * 31000 + 1 * 15000, SDS-PAGE Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.12 8
-
-
 Methanothermobacter thermautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.12 pterin contains molybdopterin guanine dinucleotide as pterin cofactor Methanothermobacter thermautotrophicus