Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ghareib, M.; Youssef, K.A.; Nour el Dein, M.M.
    Isolation and characterization of intracellular phytase from Macrophormina physeolina (1988), Zentralbl. Mikrobiol., 143, 397-403.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 2,4-dinitrophenol
-
 Macrophomina phaseolina
3.1.3.8 arsenate
-
 Macrophomina phaseolina
3.1.3.8 arsenite
-
 Macrophomina phaseolina
3.1.3.8 azide
-
 Macrophomina phaseolina
3.1.3.8 EDTA
-
 Macrophomina phaseolina
3.1.3.8 F-
-
 Macrophomina phaseolina
3.1.3.8 Hg2+
-
 Macrophomina phaseolina
3.1.3.8 iodoacetic acid
-
 Macrophomina phaseolina
3.1.3.8 phloridzin
-
 Macrophomina phaseolina
3.1.3.8 Tartrate
-
 Macrophomina phaseolina
3.1.3.8 Zn2+
-
 Macrophomina phaseolina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.91
-
 myo-inositol hexakisphosphate
-
 Macrophomina phaseolina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.8 Ca2+ activates Macrophomina phaseolina
3.1.3.8 K+ activates Macrophomina phaseolina
3.1.3.8 Zn2+ activates Macrophomina phaseolina

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.3.8 additional information high resistance to the denaturing effect of organic solvents Macrophomina phaseolina

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Macrophomina phaseolina
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Macrophomina phaseolina
-
 tassi
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8
-
 Macrophomina phaseolina

Storage Stability

EC Number Storage Stability Organism
3.1.3.8 4°C, 0.05 M Tris-HCL buffer, pH 8.2,36% loss of activity after 2 weeks Macrophomina phaseolina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Macrophomina phaseolina 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 35
-
-
 Macrophomina phaseolina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 45
-
 60 min, about 95% loss of activity Macrophomina phaseolina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5.6
-
-
 Macrophomina phaseolina

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.8 5.5 7 10 min, stable Macrophomina phaseolina