Literature summary extracted from

  • Shane, B.
    Folylpoly-gamma-glutamate synthetase (1986), Chem. Biol. Pteridines, Proc. Int. Symp. Pteridines Folic Acid Deriv. Chem. Biol. Clin. Aspects, , 719-728.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.2.17 Reducing agents absolutely dependent on Sus scrofa
6.3.2.17 Reducing agents beta-mercaptoethanol or DTT Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.17 0.057
-
5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu
-
Sus scrofa
6.3.2.17 0.062
-
pteroylmonoglutamyl-Glu
-
Sus scrofa
6.3.2.17 0.0011
-
5,6,7,8-tetrahydropteroyl-Glu3 5,6,7,8-tetrahydropteroyl-Glu5 Sus scrofa
6.3.2.17 0.002
-
5,6,7,8-tetrahydropteroyl-Glu4
-
Sus scrofa
6.3.2.17 0.005
-
7,8-dihydropteroyl-Glu
-
Sus scrofa
6.3.2.17 0.0026
-
7,8-dihydropteroyl-Glu2
-
Sus scrofa
6.3.2.17 0.0022
-
10-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
Sus scrofa
6.3.2.17 0.0034
-
5,6,7,8-tetrahydropteroyl-Glu2
-
Sus scrofa
6.3.2.17 0.0027
-
5,6,7,8-tetrahydropteroyl-Glu5
-
Sus scrofa
6.3.2.17 0.0077
-
5,6,7,8-tetrahydropteroyl-Glu
-
Sus scrofa
6.3.2.17 0.054
-
methotrexate 5-methyl-5,6,7,8-tetrahydrpteroyl-Glu Sus scrofa
6.3.2.17 0.093
-
pteroylmonoglutamate
-
Sus scrofa
6.3.2.17 0.119
-
pteroyl-Glu3
-
Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.17 Mn2+
-
Corynebacterium sp.
6.3.2.17 Mg2+ required Escherichia coli
6.3.2.17 Mg2+ required Sus scrofa
6.3.2.17 Mg2+ required Corynebacterium sp.
6.3.2.17 Mg2+ required Lacticaseibacillus casei
6.3.2.17 Mn2+ activates Sus scrofa
6.3.2.17 Mn2+ activates Lacticaseibacillus casei
6.3.2.17 K+ required form maximal activity Escherichia coli
6.3.2.17 K+ required form maximal activity Sus scrofa
6.3.2.17 K+ required form maximal activity Corynebacterium sp.
6.3.2.17 K+ required form maximal activity Lacticaseibacillus casei
6.3.2.17 K+ absolutely dependent on monovalent cation, K+ is most effective Escherichia coli
6.3.2.17 K+ absolutely dependent on monovalent cation, K+ is most effective Sus scrofa
6.3.2.17 K+ absolutely dependent on monovalent cation, K+ is most effective Corynebacterium sp.
6.3.2.17 K+ absolutely dependent on monovalent cation, K+ is most effective Lacticaseibacillus casei
6.3.2.17 K+ maximal activity at 200 mM Escherichia coli
6.3.2.17 K+ maximal activity at 200 mM Sus scrofa
6.3.2.17 K+ maximal activity at 200 mM Corynebacterium sp.
6.3.2.17 K+ maximal activity at 200 mM Lacticaseibacillus casei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.17 66000
-
gel filtration Sus scrofa
6.3.2.17 62000
-
1 * 62000, SDS-PAGE Sus scrofa
6.3.2.17 47000
-
1 * 47000, SDS-PAGE Escherichia coli
6.3.2.17 51000
-
gel filtration Corynebacterium sp.
6.3.2.17 43000
-
gel filtration Lacticaseibacillus casei
6.3.2.17 46000
-
gel filtration Escherichia coli
6.3.2.17 53000
-
1 * 53000, SDS-PAGE Corynebacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.17 Corynebacterium sp.
-
-
-
6.3.2.17 Escherichia coli
-
-
-
6.3.2.17 Lacticaseibacillus casei
-
-
-
6.3.2.17 Sus scrofa
-
hog
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.17 liver
-
Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.17 additional information enzyme also posseses dihydrofolate synthetase activity Escherichia coli ?
-
?
6.3.2.17 additional information enzyme also posseses dihydrofolate synthetase activity Corynebacterium sp. ?
-
?
6.3.2.17 additional information no dihydrofolate synthetase activity Sus scrofa ?
-
?
6.3.2.17 additional information no dihydrofolate synthetase activity Lacticaseibacillus casei ?
-
?
6.3.2.17 ATP + methotrexate + Glu
-
Sus scrofa ADP + phosphate + methotrexyl-Glu
-
?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 3 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 3 Lacticaseibacillus casei ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 5 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 2 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 2 Lacticaseibacillus casei ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Corynebacterium sp. ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Lacticaseibacillus casei ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 4 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
6.3.2.17 ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
Sus scrofa ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
6.3.2.17 ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
Corynebacterium sp. ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
6.3.2.17 ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
Lacticaseibacillus casei ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
6.3.2.17 ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
Lacticaseibacillus casei ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
6.3.2.17 ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n: 1 Sus scrofa ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
6.3.2.17 ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu n: 1 Corynebacterium sp. ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
6.3.2.17 ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
-
Escherichia coli ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
?
6.3.2.17 ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
-
Sus scrofa ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
?
6.3.2.17 ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
-
Corynebacterium sp. ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
?
6.3.2.17 ATP + 7,8-dihydropteroate + Glu
-
Sus scrofa ADP + phosphate + 7,8-dihydropteroyl-Glu
-
?
6.3.2.17 ATP + 7,8-dihydropteroyl-Glun + Glu n: 2 Sus scrofa ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
?
6.3.2.17 ATP + 7,8-dihydropteroyl-Glun + Glu n: 1 Sus scrofa ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
?
6.3.2.17 ATP + 7,8-dihydropteroyl-Glun + Glu n: 1 Lacticaseibacillus casei ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
?
6.3.2.17 ATP + pteroyl-Glu + Glu n: 3 Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
?
6.3.2.17 ATP + pteroyl-Glu + Glu n: 2 Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
?
6.3.2.17 ATP + pteroyl-Glu + Glu n: 2 Lacticaseibacillus casei ADP + phosphate + pteroylmonoglutamyl-Glu
-
?
6.3.2.17 ATP + pteroyl-Glu + Glu n: 1, i.e. pteroylmonoglutamate, folic acid Sus scrofa ADP + phosphate + pteroylmonoglutamyl-Glu
-
?
6.3.2.17 ATP + pteroyl-Glu + Glu n: 1, i.e. pteroylmonoglutamate, folic acid Lacticaseibacillus casei ADP + phosphate + pteroylmonoglutamyl-Glu
-
?
6.3.2.17 ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
-
Sus scrofa ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2
-
?
6.3.2.17 ATP + pteroyl-Glu3 + L-glutamate
-
Sus scrofa ADP + phosphate + pteroyl-Glu4
-
?
6.3.2.17 ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
-
Sus scrofa ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu2
-
?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glu3 + L-glutamate
-
Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu4
-
?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glu4 + L-glutamate
-
Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu5
-
?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glu5 + L-glutamate
-
Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu6
-
?

Subunits

EC Number Subunits Comment Organism
6.3.2.17 monomer 1 * 62000, SDS-PAGE Sus scrofa
6.3.2.17 monomer 1 * 53000, SDS-PAGE Corynebacterium sp.
6.3.2.17 monomer 1 * 43000, SDS-PAGE Lacticaseibacillus casei
6.3.2.17 monomer 1 * 47000, SDS-PAGE Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.17 additional information
-
additional information
-
Escherichia coli
6.3.2.17 additional information
-
additional information
-
Sus scrofa
6.3.2.17 additional information
-
additional information
-
Corynebacterium sp.
6.3.2.17 additional information
-
additional information
-
Lacticaseibacillus casei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.17 9.4
-
-
Escherichia coli
6.3.2.17 9.4
-
-
Sus scrofa
6.3.2.17 9.5
-
-
Sus scrofa
6.3.2.17 9.5
-
-
Corynebacterium sp.
6.3.2.17 9.5
-
-
Lacticaseibacillus casei