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Literature summary extracted from

  • Cichowicz, D.J.; Shane, B.
    Mammalian folylpoly-gamma-glutamate synthetase. 1. Purification and general properties of the hog liver enzyme (1987), Biochemistry, 26, 504-512.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.2.17 2-mercaptoethanol maximal stimulation at 25 mM Sus scrofa
6.3.2.17 2-mercaptoethanol inhbibition above 100 mM Sus scrofa
6.3.2.17 dithiothreitol maximal stimulation at 2.5 mM Sus scrofa
6.3.2.17 Reducing agents absolutely dependent on Sus scrofa
6.3.2.17 Reducing agents beta-mercaptoethanol or DTT Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.17 K+ required at low concentration, inhibition at high concentration Sus scrofa
6.3.2.17 NH4+ required at low concentration, inhibition at high concentration Sus scrofa
6.3.2.17 Rb+ required at low concentration, inhibition at high concentration Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.17 0.01
-
 ATP ATP Sus scrofa
6.3.2.17 0.332
-
 Glu
-
 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.17 K+ absolutely dependent on monovalent cation, K+ is most effective Sus scrofa
6.3.2.17 K+ inhibition at high concentrations Sus scrofa
6.3.2.17 Mg2+ required Sus scrofa
6.3.2.17 Mg2+ in presence of 0.1 mM ATP, maximal activity at 2 mM Mg2+ Sus scrofa
6.3.2.17 Mn2+ supports activity, at 1 mM 68% of the activity obtained with Mg2+ Sus scrofa
6.3.2.17 NH4+ stimulates to a lesser extent than K+ Sus scrofa
6.3.2.17 NH4+ inhibition by high concentrations Sus scrofa
6.3.2.17 Rb+ absolute requirement for monovalent cation is met by, inhibition at high concentrations Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.17 Sus scrofa
-
 hog
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.17 two forms Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.17 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.17 0.465
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Sus scrofa ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
6.3.2.17 ATP + 7,8-dihydropteroate + Glu
-
 Sus scrofa ADP + phosphate + 7,8-dihydropteroyl-Glu
-
 ?
6.3.2.17 ATP + 7,8-dihydropteroyl-Glun + Glu n: 1 Sus scrofa ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
 ?
6.3.2.17 ATP + aminopterin + Glu
-
 Sus scrofa ADP + phosphate + aminopteryl-Glu
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.17 9.4
-
-
 Sus scrofa