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Literature summary extracted from

  • Shiota, T.; Baugh, C.M.; Jackson, R.; Dillard, R.
    The enzymatic synthesis of hydroxymethyldihydropteridine pyrophosphate and dihydrofolate (1969), Biochemistry, 8, 5022-5028.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.15 p-aminobenzoylglutamate weak Lactiplantibacillus plantarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.15 Mg2+ required to synthesize product Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate Lactiplantibacillus plantarum
-
 diphosphate + 7,8-dihydropteroate
-
 ?
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoylglutamate Lactiplantibacillus plantarum
-
 diphosphate + dihydrofolate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.15 Lactiplantibacillus plantarum
-
-
-
6.3.2.12 Lactiplantibacillus plantarum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.15 precipitation with ammonium sulfate followed by column chromatography Lactiplantibacillus plantarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate
-
 Lactiplantibacillus plantarum diphosphate + 7,8-dihydropteroate
-
 ?
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoylglutamate
-
 Lactiplantibacillus plantarum diphosphate + dihydrofolate
-
 ?
6.3.2.12 ATP + 7,8-dihydropteroate + L-Glu
-
 Lactiplantibacillus plantarum ADP + phosphate + 7,8-dihydrofolate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.15 60
-
 10 min, loss of activity Lactiplantibacillus plantarum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.15 1
-
 p-aminobenzoylglutamate
-
 Lactiplantibacillus plantarum