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Literature summary extracted from

  • Bognar, A.L.; Osborne, C.; Shane, B.; Singer, S.C.; Ferone, R.
    Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase. Cloning and high expression of the Escherichia coli folC gene and purification and properties of the gene product (1985), J. Biol. Chem., 260, 5625-5630.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.12
-
 Escherichia coli
6.3.2.17 high expression Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.12 0.0006
-
 7,8-dihydropteroate
-
 Escherichia coli
6.3.2.12 0.0069
-
 ATP
-
 Escherichia coli
6.3.2.12 0.0081
-
 ATP
-
 Escherichia coli
6.3.2.12 3.9
-
 Glu
-
 Escherichia coli
6.3.2.17 0.014
-
 10-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
 Escherichia coli
6.3.2.17 0.0665
-
 ATP reaction with 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu Escherichia coli
6.3.2.17 0.333
-
 Glu reaction with 10-formyl-5,6,7,8-tetrahydropteroyl-Glu Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.17 48000
-
 x * 48000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.12 Escherichia coli
-
-
-
6.3.2.17 Escherichia coli
-
 high expression
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.12
-
 Escherichia coli
6.3.2.17
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.12 0.535
-
-
 Escherichia coli
6.3.2.17 0.535
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.12 ATP + 7,8-dihydropteroate + L-Glu
-
 Escherichia coli ADP + phosphate + 7,8-dihydrofolate
-
 ?
6.3.2.12 ATP + 7,8-dihydropteroate + L-glutamate ATP in form of MgATP2- Escherichia coli ADP + phosphate + 7,8-dihydropteroylglutamate
-
 ?
6.3.2.17 ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
-
 Escherichia coli ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.17 ? x * 48000, SDS-PAGE Escherichia coli