BRENDA - Enzyme Database show
show all sequences of 7.6.2.8

Mechanistic basis of vitamin B12 and cobinamide salvaging by the Vibrio species

Agarwal, S.; Dey, S.; Ghosh, B.; Biswas, M.; Dasgupta, J.; Biochim. Biophys. Acta 1867, 140-151 (2019)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene btuF, phylogenetic tree of BtuF, recombinant expression of His6-tagged BtuF in Escherichia coli strain XL1-Blue
Vibrio cholerae serotype O1
Crystallization (Commentary)
Crystallization
Organism
vitamin B12-bound VcBtuF, protein in a solution with vitamin B12 in a 3:1 ratio, and 50 mM Tris-HCl, pH 7.0, and 300 mM NaCl, mixing of 0.003 ml of protein solution with 0.002 ml of precipitant solution containing 0.8 M ammonium sulfate, 0.1 M Tris, pH 8.0, and equilibration against a reservoir solution containing 0.5 ml of 1.6 M ammonium sulfate, 0.1 M HEPES, pH 7.0, 20C, 7 days, hanging drop vapour diffusion method, X-ray diffraction structure determination and analysis at 1.67 A resolution
Vibrio cholerae serotype O1
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
transmembrane enzyme
Vibrio cholerae serotype O1
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Vibrio cholerae serotype O1
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1
-
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1 Classical Ogawa 395
-
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1 ATCC 39541
-
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1 O395
-
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1
-
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1 Classical Ogawa 395
-
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1 ATCC 39541
-
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1 O395
-
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1 Classical Ogawa 395
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1 ATCC 39541
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1 O395
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
additional information
Vibrio cholerae serotype O1
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
?
-
-
-
additional information
Vibrio cholerae serotype O1 Classical Ogawa 395
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
?
-
-
-
additional information
Vibrio cholerae serotype O1 ATCC 39541
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
?
-
-
-
additional information
Vibrio cholerae serotype O1 O395
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Vibrio cholerae serotype O1
A0A0H3AMA6 AND A5F1V0 AND A5F5P5
genes btuC, btuD, and btuF encoding for vitamin B12 import system permease protein BtuC, vitamin B12 import ATP-binding protein BtuD, and vitamin B12-binding protein BtuF
-
Vibrio cholerae serotype O1 ATCC 39541
A0A0H3AMA6 AND A5F1V0 AND A5F5P5
genes btuC, btuD, and btuF encoding for vitamin B12 import system permease protein BtuC, vitamin B12 import ATP-binding protein BtuD, and vitamin B12-binding protein BtuF
-
Vibrio cholerae serotype O1 Classical Ogawa 395
A0A0H3AMA6 AND A5F1V0 AND A5F5P5
genes btuC, btuD, and btuF encoding for vitamin B12 import system permease protein BtuC, vitamin B12 import ATP-binding protein BtuD, and vitamin B12-binding protein BtuF
-
Vibrio cholerae serotype O1 O395
A0A0H3AMA6 AND A5F1V0 AND A5F5P5
genes btuC, btuD, and btuF encoding for vitamin B12 import system permease protein BtuC, vitamin B12 import ATP-binding protein BtuD, and vitamin B12-binding protein BtuF
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1 Classical Ogawa 395
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1 ATCC 39541
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1 O395
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1 Classical Ogawa 395
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1 ATCC 39541
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1 O395
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1 Classical Ogawa 395
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1 ATCC 39541
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1 O395
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
additional information
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
749978
Vibrio cholerae serotype O1
?
-
-
-
-
additional information
VcBtuF shows a distinctive phenomenon of heme binding with comparable affinity to vitamin B12
749978
Vibrio cholerae serotype O1
?
-
-
-
-
additional information
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
749978
Vibrio cholerae serotype O1 Classical Ogawa 395
?
-
-
-
-
additional information
VcBtuF shows a distinctive phenomenon of heme binding with comparable affinity to vitamin B12
749978
Vibrio cholerae serotype O1 Classical Ogawa 395
?
-
-
-
-
additional information
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
749978
Vibrio cholerae serotype O1 ATCC 39541
?
-
-
-
-
additional information
VcBtuF shows a distinctive phenomenon of heme binding with comparable affinity to vitamin B12
749978
Vibrio cholerae serotype O1 ATCC 39541
?
-
-
-
-
additional information
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
749978
Vibrio cholerae serotype O1 O395
?
-
-
-
-
additional information
VcBtuF shows a distinctive phenomenon of heme binding with comparable affinity to vitamin B12
749978
Vibrio cholerae serotype O1 O395
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
20
-
BtuF binding assay at
Vibrio cholerae serotype O1
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
BtuF binding assay at
Vibrio cholerae serotype O1
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Vibrio cholerae serotype O1
Cloned(Commentary) (protein specific)
Commentary
Organism
gene btuF, phylogenetic tree of BtuF, recombinant expression of His6-tagged BtuF in Escherichia coli strain XL1-Blue
Vibrio cholerae serotype O1
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Vibrio cholerae serotype O1
Crystallization (Commentary) (protein specific)
Crystallization
Organism
vitamin B12-bound VcBtuF, protein in a solution with vitamin B12 in a 3:1 ratio, and 50 mM Tris-HCl, pH 7.0, and 300 mM NaCl, mixing of 0.003 ml of protein solution with 0.002 ml of precipitant solution containing 0.8 M ammonium sulfate, 0.1 M Tris, pH 8.0, and equilibration against a reservoir solution containing 0.5 ml of 1.6 M ammonium sulfate, 0.1 M HEPES, pH 7.0, 20C, 7 days, hanging drop vapour diffusion method, X-ray diffraction structure determination and analysis at 1.67 A resolution
Vibrio cholerae serotype O1
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
transmembrane enzyme
Vibrio cholerae serotype O1
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Vibrio cholerae serotype O1
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1
-
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1 Classical Ogawa 395
-
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1 ATCC 39541
-
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1 O395
-
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1
-
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1 Classical Ogawa 395
-
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1 ATCC 39541
-
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1 O395
-
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1 Classical Ogawa 395
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1 ATCC 39541
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
Vibrio cholerae serotype O1 O395
-
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
?
additional information
Vibrio cholerae serotype O1
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
?
-
-
-
additional information
Vibrio cholerae serotype O1 Classical Ogawa 395
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
?
-
-
-
additional information
Vibrio cholerae serotype O1 ATCC 39541
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
?
-
-
-
additional information
Vibrio cholerae serotype O1 O395
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1 Classical Ogawa 395
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1 ATCC 39541
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1 O395
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1 Classical Ogawa 395
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1 ATCC 39541
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1 O395
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1 Classical Ogawa 395
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1 ATCC 39541
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
-
749978
Vibrio cholerae serotype O1 O395
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
additional information
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
749978
Vibrio cholerae serotype O1
?
-
-
-
-
additional information
VcBtuF shows a distinctive phenomenon of heme binding with comparable affinity to vitamin B12
749978
Vibrio cholerae serotype O1
?
-
-
-
-
additional information
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
749978
Vibrio cholerae serotype O1 Classical Ogawa 395
?
-
-
-
-
additional information
VcBtuF shows a distinctive phenomenon of heme binding with comparable affinity to vitamin B12
749978
Vibrio cholerae serotype O1 Classical Ogawa 395
?
-
-
-
-
additional information
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
749978
Vibrio cholerae serotype O1 ATCC 39541
?
-
-
-
-
additional information
VcBtuF shows a distinctive phenomenon of heme binding with comparable affinity to vitamin B12
749978
Vibrio cholerae serotype O1 ATCC 39541
?
-
-
-
-
additional information
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
749978
Vibrio cholerae serotype O1 O395
?
-
-
-
-
additional information
VcBtuF shows a distinctive phenomenon of heme binding with comparable affinity to vitamin B12
749978
Vibrio cholerae serotype O1 O395
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
20
-
BtuF binding assay at
Vibrio cholerae serotype O1
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
BtuF binding assay at
Vibrio cholerae serotype O1
General Information
General Information
Commentary
Organism
additional information
molecular dynamics simulation of structure of the cobalamin-binding protein BtuF compared to Escherichia coli BtuF structure
Vibrio cholerae serotype O1
General Information (protein specific)
General Information
Commentary
Organism
additional information
molecular dynamics simulation of structure of the cobalamin-binding protein BtuF compared to Escherichia coli BtuF structure
Vibrio cholerae serotype O1
Other publictions for EC 7.6.2.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749978
Agarwal
Mechanistic basis of vitamin ...
Vibrio cholerae serotype O1, Vibrio cholerae serotype O1 ATCC 39541, Vibrio cholerae serotype O1 Classical Ogawa 395, Vibrio cholerae serotype O1 O395
Biochim. Biophys. Acta
1867
140-151
2019
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1
1
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1
1
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16
-
8
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20
-
1
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1
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-
1
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1
1
1
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1
1
-
16
-
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-
-
-
20
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
749821
Okamoto
Characterization of human ATP ...
Homo sapiens
Biochem. Biophys. Res. Commun.
496
1122-1127
2018
-
-
1
-
-
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1
-
2
1
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1
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3
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1
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1
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2
-
1
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1
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1
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1
1
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-
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1
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2
1
-
1
-
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-
1
1
-
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
750412
Santos
Functional and structural cha ...
Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842, Lactobacillus delbrueckii subsp. bulgaricus DSM 20081, Lactobacillus delbrueckii subsp. bulgaricus JCM 1002, Lactobacillus delbrueckii subsp. bulgaricus NBRC 13953, Lactobacillus delbrueckii subsp. bulgaricus NCIMB 11778
eLife
7
e35828
2018
-
-
1
1
-
-
6
1
1
1
-
12
-
8
-
-
-
-
-
-
-
-
18
1
1
-
-
-
1
-
-
1
-
-
-
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-
1
1
1
-
-
-
6
-
1
1
1
-
12
-
-
-
-
-
-
-
-
18
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
751644
Schmitt
Vitamin B12 import is all abo ...
Escherichia coli
Nat. Chem. Biol.
14
640-641
2018
-
-
-
-
-
-
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1
1
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1
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1
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1
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1
1
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1
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
751670
Rempel
Cysteine-mediated decyanation ...
Thiobacillus denitrificans
Nat. Commun.
9
3038
2018
-
-
1
1
-
-
-
-
1
1
-
2
-
1
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1
-
-
-
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4
-
1
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1
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1
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1
1
1
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1
1
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2
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1
-
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-
-
4
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
751661
Goudsmits
Single-molecule visualization ...
Escherichia coli
Nat. Commun.
8
1652
2017
-
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1
-
5
1
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2
1
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1
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1
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1
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2
1
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5
1
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1
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2
1
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3
3
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-
752215
Mireku
Structural basis of nanobody- ...
Escherichia coli
Sci. Rep.
7
14296
2017
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1
1
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1
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1
1
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1
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1
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1
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1
1
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752221
Mireku
Conformational change of a tr ...
Escherichia coli
Sci. Rep.
7
41575
2017
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1
1
8
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1
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2
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1
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3
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1
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1
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1
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1
1
1
8
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1
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2
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1
1
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3
-
1
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-
-
1
-
-
-
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3
3
-
-
-
750063
Priess
Release of entropic spring re ...
Escherichia coli
Biophys. J.
110
2407-2418
2016
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1
1
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1
1
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1
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2
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1
1
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1
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1
1
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1
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-
1
1
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2
2
-
-
-
751956
Pan
ATP hydrolysis induced confor ...
Escherichia coli
PLoS ONE
11
e0166980
2016
-
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2
1
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1
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1
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1
1
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1
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1
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2
1
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1
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-
1
1
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2
2
-
-
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752200
Kawaguchi
Translocation of the ABC tran ...
Homo sapiens
Sci. Rep.
6
30183
2016
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1
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1
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3
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1
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3
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1
2
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2
2
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734003
Su
Conformational motions and fun ...
Escherichia coli
Int. J. Mol. Sci.
16
17933-17951
2015
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2
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1
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734281
Joseph
Conformational cycle of the vi ...
Escherichia coli
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289
3176-3185
2014
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1
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1
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734760
Korkhov
Structure of AMP-PNP-bound Btu ...
Escherichia coli
Nat. Struct. Mol. Biol.
21
1097-1099
2014
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1
1
1
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1
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1
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1
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734820
Gopinath
A vitamin B12 transporter in M ...
Mycobacterium tuberculosis
Open Biology
3
120175
2013
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2
2
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719509
Korkhov
Asymmetric states of vitamin B ...
Escherichia coli
FEBS Lett.
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972-976
2012
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1
1
1
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2
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2
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720849
Weng
-
The conformational transition ...
Escherichia coli
PLoS ONE
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2012
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734771
Korkhov
Structure of AMP-PNP-bound vit ...
Escherichia coli
Nature
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367-372
2012
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1
2
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1
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719930
Di Bartolo
In vitro folding and assembly ...
Escherichia coli
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719987
Joseph
Transmembrane gate movements i ...
Escherichia coli
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2011
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Lewinson
A distinct mechanism for the A ...
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713485
Kandt
Holo-BtuF stabilizes the open ...
Escherichia coli
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2010
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713505
Sun
Molecular dynamics simulation ...
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620-630
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696776
Weng
Asymmetric conformational flex ...
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697950
Goetz
Distinct gate conformations of ...
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685614
Liu
Study on the mechanism of the ...
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685626
Weng
The conformational coupling an ...
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Ivetac
Dynamics and function in a bac ...
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2767-2778
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Sonne
Simulation of the coupling bet ...
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