Cloned (Comment) | Organism |
---|---|
genes btuC and btuD form a cluster, encoding permease protein btuC and ATP-binding protein btuD, forming the ABC transporter BtuCD, expression of wild-type and utant enzymes as His-tagged proteins | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
crystal structure analysis of the crystal structure of the BtuCD-F complex, modelling, overview | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
S141C | site-directed mutagenesis, structure analysis | Escherichia coli |
S143C | site-directed mutagenesis, structure analysis | Escherichia coli |
T168C | site-directed mutagenesis, structure analysis | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | periplasmic and cytosolic orientation | Escherichia coli | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + vitamin B12/out | Escherichia coli | BtuCD is a type II ABC importer that catalyzes the translocation of vitamin B12 from the periplasm into the cytoplasm of Escherichia coli. BtuD is complexed with BtuC, a permease protein, and BtuF, a periplasmic binding protein, structure, overview | ADP + phosphate + vitamin B12/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P06611 | BtuD; gene btuD | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes by nickel affinity chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + vitamin B12/out | - |
Escherichia coli | ADP + phosphate + vitamin B12/in | - |
? | |
ATP + H2O + vitamin B12/out | BtuCD is a type II ABC importer that catalyzes the translocation of vitamin B12 from the periplasm into the cytoplasm of Escherichia coli. BtuD is complexed with BtuC, a permease protein, and BtuF, a periplasmic binding protein, structure, overview | Escherichia coli | ADP + phosphate + vitamin B12/in | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | crystal structure analysis for conformations of the transmembrane domains that form inner and outer gates, structure comparison, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
BtuCD | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |