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Literature summary for 7.5.2.9 extracted from

  • Horler, R.; Mueller, A.; Williamson, D.; Potts, J.; Wilson, K.; Thomas, G.
    Furanose-specific sugar transport. Characterization of a bacterial galactofuranose-binding protein (2009), J. Biol. Chem., 284, 31156-31163 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.5.2.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + D-galactofuranose-[galactofuranose-binding protein][side 1] Escherichia coli
-
 ADP + phosphate + D-galactofuranose[side 2] + [galactofuranose-binding protein][side 1]
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P39325
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and DEAE-Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + D-galactofuranose-[galactofuranose-binding protein][side 1]
-
 Escherichia coli ADP + phosphate + D-galactofuranose[side 2] + [galactofuranose-binding protein][side 1]
-
 ?
ATP + H2O + D-galactofuranose-[galactofuranose-binding protein][side 1] the enzyme shows selective binding of galactofuranose over galactopyranose Escherichia coli ADP + phosphate + D-galactofuranose[side 2] + [galactofuranose-binding protein][side 1]
-
 ?
ATP + H2O + D-galactopyranose-[galactopyranose-binding protein][side 1] the enzyme shows selective binding of galactofuranose over galactopyranose Escherichia coli ADP + phosphate + D-galactopyranose[side 2] + [galactopyranose-binding protein][side 1]
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
YtfQ substrate binding protein of the galactopyranose transporter Escherichia coli