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Literature summary for 7.4.2.5 extracted from

  • Hammes, U.; Meier, S.; Dietrich, D.; Ward, J.; Rentsch, D.
    Functional properties of the Arabidopsis peptide transporters AtPTR1 and AtPTR5 (2010), J. Biol. Chem., 285, 39710-39717.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.4.2.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Xenopus laevis oocyte Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Carbonyl cyanide m-chlorophenylhydrazone 10 mM, 20% residual activity; 10 mM, 26% residual activity Arabidopsis thaliana
diethyl dicarbonate 10 mM, 0.4% residual activity; 10 mM, 0.6% residual activity Arabidopsis thaliana
L-Phe-L-Ala in the absence of dipeptides, transporter shows proton-dependent leak currents that are inhibited by Phe-Ala; in the absence of dipeptides, transporter shows proton-dependent leak currents that are inhibited by Phe-Ala, Trp-Ala, and Phe-Phe Arabidopsis thaliana
L-Phe-L-Phe in the absence of dipeptides, transporter shows proton-dependent leak currents that are inhibited by Phe-Ala, Trp-Ala, and Phe-Phe. Phe-Ala reduces leak currents by binding to the substrate-binding site with a high apparent affinity Arabidopsis thaliana
L-Trp-L-Ala in the absence of dipeptides, transporter shows proton-dependent leak currents that are inhibited by Phe-Ala, Trp-Ala, and Phe-Phe Arabidopsis thaliana
N-ethylmaleimide 10 mM, 12% residual activity; 10 mM, 14% residual activity Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LFB8 isoform PTR5
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Arabidopsis thaliana Q9M390 isoform PTR1
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + L-Ala-L-Ala/in K0.5 value 0.023 mM at -140 mV Arabidopsis thaliana ADP + phosphate + L-Ala-L-Ala/out
-
 ?
ATP + H2O + L-Ala-L-Ala/in K0.5 value 0.057 mM at -140 mV Arabidopsis thaliana ADP + phosphate + L-Ala-L-Ala/out
-
 ?
ATP + H2O + L-Ala-L-Asp/in K0.5 value 0.058 mM at -140 mV Arabidopsis thaliana ADP + phosphate + L-Ala-L-Asp/out
-
 ?
ATP + H2O + L-Ala-L-Asp/in K0.5 value 0.129 mM at -140 mV Arabidopsis thaliana ADP + phosphate + L-Ala-L-Asp/out
-
 ?
ATP + H2O + L-Ala-L-Lys/in K0.5 value 0.142 mM at -140 mV Arabidopsis thaliana ADP + phosphate + L-Ala-L-Lys/out
-
 ?
ATP + H2O + L-Ala-L-Lys/in K0.5 value 0.167 mM at -140 mV Arabidopsis thaliana ADP + phosphate + L-Ala-L-Lys/out
-
 ?
additional information dipeptide transport by AtPTR1 is electrogenic and dependent on protons but not sodium. Transport activity is voltage-dependent, and currents increase supralinearly with more negative membrane potentials and do not saturate. The voltage dependence of the apparent affinities differes between Ala-Ala, Ala-Lys, and Ala-Asp and is not conserved between the two transporters AtPTR1 and AtPTR5. The apparent affinity of AtPTR1 for these dipeptides is pH-dependent and decreases with decreasing proton concentration Arabidopsis thaliana ?
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 ?
additional information dipeptide transport by AtPTR5 is electrogenic and dependent on protons but not sodium. Transport activity is voltage-dependent, and currents increase supralinearly with more negative membrane potentials and do not saturate. The voltage dependence of the apparent affinities differes between Ala-Ala, Ala-Lys, and Ala-Asp and is not conserved between the two transporters AtPTR1 and AtPTR5 Arabidopsis thaliana ?
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 ?

Synonyms

Synonyms Comment Organism
PTR1
-
 Arabidopsis thaliana