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Literature summary for 7.4.2.5 extracted from
- Engineering ATPase activity in the isolated ABC cassette of human TAP1 (2006), J. Biol. Chem., 281, 27471-27480.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the coding sequence of the nucleotide-binding domain of TAP1, amino acids 489-748, is cloned into the pET21a+ vector | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D668E | mutation in the nucleotide-binding domain of TAP1 | Homo sapiens |
| D668E/Q710H | mutation in the nucleotide-binding domain of TAP1 | Homo sapiens |
| Q710H | mutation in the nucleotide-binding domain of TAP1 | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.174 | - |
ATP | mutant Q701H | Homo sapiens |  |
| 0.18 | - |
ATP | mutant D668/Q701H | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum membrane | - |
Homo sapiens | 5789 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
nucleotide-binding domain of TAP1, determined by SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + peptide/in | Homo sapiens | - |
ADP + phosphate + peptide/out | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a Zn2+-iminodiacetic acid column and a Superdex 200HR 26/60 column | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + peptide/in | - |
Homo sapiens | ADP + phosphate + peptide/out | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | TAP1, TAP2 | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| peptide-transporting ATPase | - |
Homo sapiens |
| TAP | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
ATPase activity assay | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
ATP | mutant Q701H | Homo sapiens | |
| 0.001 | - |
ATP | mutant D668/Q701H | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
ATPase activity assay | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | mutant Q701H | Homo sapiens |
| 6 | 7 | mutant D668/Q701H | Homo sapiens |
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Release 2023.1 (January 2023)
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