Literature summary for 7.4.2.14 extracted from

Bahram, S.; Arnold, D.; Bresnahan, M.; Strominger, J.; Spies, T.
Two putative subunits of a peptide pump encoded in the human major histocompatibility complex class II region (1991), Proc. Natl. Acad. Sci. USA, 88, 10094-10098 .

Cloned(Commentary)

Cloned (Comment)	Organism
genes TAP1 and TAP2, or PSF1 and PSF2, DNA and amino acid sequence determination and analysis, PSF2 is identified 10 kb from PSFI, near the class I DOB gene, sequence comparisons	Homo sapiens

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum membrane	TAP1 and TAP2 contain a hydrophobic domain with a tentative number of eight membrane-spanning segments. Location of PSF1 is in the ER membrane, with the hydrophilic ATP-binding fold facing the cytosol. Potential N-glycosylation sites likely to be exposed to the ER lumen are present in PSF1 at the positions 250, 279, and 302 but are missing in PSF2	Homo sapiens	5789	-
additional information	enzyme membrane topology, hydropathy profiles of PSF2 and PSF1, overview	Homo sapiens	-	-

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + antigen peptide[side 1]	Homo sapiens	-	ADP + phosphate + antigen peptide[side 2]	-	?

Organism	UniProt	Comment	Textmining
Homo sapiens	Q03518 AND Q03519	TAP1 and TAP2 subunits	-

Posttranslational Modification	Comment	Organism
glycoprotein	potential N-glycosylation sites likely to be exposed to the ER lumen are present in PSF1 at the positions 250, 279, and 302 but are missing in PSF2	Homo sapiens

Source Tissue	Comment	Organism	Textmining
B-lymphocyte	-	Homo sapiens	-
carcinoma cell	-	Homo sapiens	-
epithelial cell	-	Homo sapiens	-
fibroblast	-	Homo sapiens	-
G-292 cell	-	Homo sapiens	-
HeLa cell	-	Homo sapiens	-
HL-60 cell	-	Homo sapiens	-
HPB-ALL cell	-	Homo sapiens	-
HT-1080 cell	-	Homo sapiens	-
intestine-407 cell	-	Homo sapiens	-
LCL721 cell	-	Homo sapiens	-
MOLT-13 cell	-	Homo sapiens	-
MOLT-4 cell	-	Homo sapiens	-
monocyte	-	Homo sapiens	-
additional information	TAP1 and TAP2 expression analysis and profiles, overview	Homo sapiens	-
MRC-5 cell	-	Homo sapiens	-
promyelocyte	-	Homo sapiens	-
RAJI cell	-	Homo sapiens	-
T-lymphocyte	-	Homo sapiens	-
U-937 cell	-	Homo sapiens	-

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + antigen peptide[side 1]	-	Homo sapiens	ADP + phosphate + antigen peptide[side 2]	-	?

Subunits	Comment	Organism
heterodimer	-	Homo sapiens

Synonyms	Comment	Organism
antigen peptide transporter 2	UniProt	Homo sapiens
APT2	UniProt	Homo sapiens
peptide pump	-	Homo sapiens
peptide supply factor	-	Homo sapiens
PSF1	-	Homo sapiens
PSF2	-	Homo sapiens
TAP	-	Homo sapiens
TAP1	-	Homo sapiens
TAP2	-	Homo sapiens

Cofactor	Comment	Organism	Structure
ATP	both, TAP1 and TAP2, contain a highly conserved ATP-binding fold	Homo sapiens

General Information	Comment	Organism
evolution	the peptide supply factors, PSF1 and PSF2, are members of the multidrug-resistance family of transporters. They share 25% homology in a hydrophobic domain with a tentative number of eight membrane-spanning segments. The C-terminal 25 amino acids are entirely unrelated. close evolutionary relationship between PSF1 and PSF2 (TAP1 and TAP2)	Homo sapiens
malfunction	some allelic variants of PSFI and/or PSF2 can restrict antigen presentation and are related to several autoimmune diseases that are associated with genes in the MHC class II region	Homo sapiens
physiological function	the peptide supply factors, PSF1 and PSF2, are members of the multidrug-resistance family of transporters and may pump cytosolic peptides into the membrane-bound compartment where class I molecules assemble. Coregulation of PSFI and PSF2 by gamma interferon. They have a common role in peptide-loading of class I molecules	Homo sapiens