Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.3.2.7 extracted from

  • Hemmingsson, O.; Zhang, Y.; Still, M.; Naredi, P.
    ASNA1, an ATPase targeting tail-anchored proteins, regulates melanoma cell growth and sensitivity to cisplatin and arsenite (2009), Cancer Chemother. Pharmacol., 63, 491-499.
    View publication on PubMed
Search for more literature for 7.3.2.7

Cloned(Commentary)

Cloned (Comment) Organism
transfection of T-289 melanoma cells with either ASNA1 sense or ASNA1 antisense constructs, expression analysis Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information T-289 clones with siRNA decreased ASNA1 expression exhibit 51% longer doubling times than wild-type T-289 cells. After exposure to cisplatin, ASNA1 downregulated cells display a significant increase in apoptosis. The cisplatin IC50 in ASNA1 underexpressing cells is 41.7% of the IC50 for wild-type cells, and the arsenite IC50 is 59.9% of wild-type IC50. Phenotypes, overview Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + arsenite/in Homo sapiens
-
 ADP + phosphate + arsenite/out
-
 ?
ATP + H2O + cisplatin/in Homo sapiens
-
 ADP + phosphate + cisplatin/out
-
 ?
additional information Homo sapiens ASNA1, an ATPase targeting tail-anchored proteins, regulates melanoma cell growth and sensitivity to cisplatin and arsenite. ASNA1 is also an essential ATPase for the insertion of tail-anchored proteins into endoplasmic reticulum membranes and a regulator of insulin secretion. ASNA1 expression is necessary for growth. ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O43681
-
-

Source Tissue

Source Tissue Comment Organism Textmining
melanoma cell
-
 Homo sapiens
-
T-289 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + arsenite/in
-
 Homo sapiens ADP + phosphate + arsenite/out
-
 ?
ATP + H2O + cisplatin/in
-
 Homo sapiens ADP + phosphate + cisplatin/out
-
 ?
additional information ASNA1, an ATPase targeting tail-anchored proteins, regulates melanoma cell growth and sensitivity to cisplatin and arsenite. ASNA1 is also an essential ATPase for the insertion of tail-anchored proteins into endoplasmic reticulum membranes and a regulator of insulin secretion. ASNA1 expression is necessary for growth. Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
ASNA1
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens