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Literature summary for 7.3.2.7 extracted from
- Role of the linker region of the anion-stimulated ATPase ArsA. Effect of deletion and point mutations in the linker region (2001), J. Biol. Chem., 276, 29582-29587.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli JM109 with a His-tag | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D303G | highly sensitive towards arsenite | Escherichia coli |
| D311G | no effect of resistance towards arsenite | Escherichia coli |
| D320G | no effect of resistance towards arsenite | Escherichia coli |
| DELTA1-277 | same arsenite resistance like wild-type when coexpressed with DELTA280-583 or DELTA324-583 | Escherichia coli |
| DELTA1-320 | no arsenite resistance in cells expressing that protein in combination with DELTA280-583 but resistance like wild-type expressing cells when expressed in combination with DELTA324-583 | Escherichia coli |
| DELTA280-583 | no arsenite resistance in cells expressing that protein in combination with DELTA1-320 but resistance like wild-type expressing cells when expressed in combination with DELTA1-277 | Escherichia coli |
| DELTA324-583 | same arsenite resistance like wild-type when coexpressed with DELTA1-320 or DELTA1-277 | Escherichia coli |
| G284S | no resistance towards arsenite | Escherichia coli |
| R290K | no effect of resistance towards arsenite | Escherichia coli |
| R290S | decreased resistance towards arsenite | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ArsA ATPase | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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